Review of CRISPR/Cas9 sgRNA design tools
The adaptive immunity system in bacteria and archaea, Clustered Regularly Interspaced
Short Palindromic Repeats, CRISPR-associate (CRISPR/Cas), has been adapted as a …
Short Palindromic Repeats, CRISPR-associate (CRISPR/Cas), has been adapted as a …
Applications of the versatile CRISPR‐Cas13 RNA targeting system
M Kordyś, R Sen, Z Warkocki - Wiley Interdisciplinary Reviews …, 2022 - Wiley Online Library
CRISPR‐Cas are adaptable natural prokaryotic defense systems that act against invading
viruses and plasmids. Among the six currently known major CRISPR‐Cas types, the type VI …
viruses and plasmids. Among the six currently known major CRISPR‐Cas types, the type VI …
A versatile toolkit for CRISPR-Cas13-based RNA manipulation in Drosophila
N Huynh, N Depner, R Larson, K King-Jones - Genome Biology, 2020 - Springer
Advances in CRISPR technology have immensely improved our ability to manipulate nucleic
acids, and the recent discovery of the RNA-targeting endonuclease Cas13 adds even further …
acids, and the recent discovery of the RNA-targeting endonuclease Cas13 adds even further …
CRISPR-DT: designing gRNAs for the CRISPR-Cpf1 system with improved target efficiency and specificity
Abstract Motivation The Clustered Regularly Interspaced Short Palindromic Repeats
(CRISPR)-Cpf1 system has been successfully applied in genome editing. However, target …
(CRISPR)-Cpf1 system has been successfully applied in genome editing. However, target …
Abrogation of PRRSV infectivity by CRISPR-Cas13b-mediated viral RNA cleavage in mammalian cells
CRISPR/Cas9 enables dsDNA viral genome engineering. However, the lack of RNA
targeting activities limits the ability of CRISPR/Cas9 to combat RNA viruses. The recently …
targeting activities limits the ability of CRISPR/Cas9 to combat RNA viruses. The recently …
Design of guide RNA for CRISPR/Cas plant genome editing
GA Gerashchenkov, NA Rozhnova, BR Kuluev… - Molecular Biology, 2020 - Springer
CRISPR/Cas technology of genome editing is a powerful tool for making targeted changes
in the DNA of various organisms, including plants. The choice of the precise nucleotide …
in the DNA of various organisms, including plants. The choice of the precise nucleotide …
Transcriptome-wide Cas13 guide RNA design for model organisms and viral RNA pathogens
The recent characterization of RNA-targeting CRISPR nucleases has enabled diverse
transcriptome engineering and screening applications that depend crucially on prediction …
transcriptome engineering and screening applications that depend crucially on prediction …
[HTML][HTML] CRISPR-Cas13a mediated targeting of hepatitis C virus internal-ribosomal entry site (IRES) as an effective antiviral strategy
Hepatitis C is an inflammatory liver disease caused by the single-stranded RNA (ssRNA)
hepatitis C virus (HCV). The genetic diversity of the virus and quasispecies produced during …
hepatitis C virus (HCV). The genetic diversity of the virus and quasispecies produced during …
[HTML][HTML] Systematic analysis, identification, and use of CRISPR/Cas13a–associated crRNAs for sensitive and specific detection of the lcrV gene of Yersinia pestis
Z Schultzhaus, Z Wang, D Stenger - Diagnostic Microbiology and Infectious …, 2021 - Elsevier
CRISPR-associated proteins that produce a signal in the presence of a target nucleic acid
represent potentially powerful tools for diagnostics, but they also exhibit shortfalls that …
represent potentially powerful tools for diagnostics, but they also exhibit shortfalls that …
Engineered CRISPR/Cas13d Sensing hTERT Selectively Inhibits the Progression of Bladder Cancer In Vitro
C Zhuang, C Zhuang, Q Zhou, X Huang… - Frontiers in Molecular …, 2021 - frontiersin.org
Aptazyme and CRISPR/Cas gene editing system were widely used for regulating gene
expression in various diseases, including cancer. This work aimed to reconstruct …
expression in various diseases, including cancer. This work aimed to reconstruct …