Industrial biotechnology of Pseudomonas putida: advances and prospects

A Weimer, M Kohlstedt, DC Volke, PI Nikel… - Applied Microbiology …, 2020 - Springer
Pseudomonas putida is a Gram-negative, rod-shaped bacterium that can be encountered in
diverse ecological habitats. This ubiquity is traced to its remarkably versatile metabolism …

Using genome-scale models to predict biological capabilities

EJ O'Brien, JM Monk, BO Palsson - Cell, 2015 - cell.com
Constraint-based reconstruction and analysis (COBRA) methods at the genome scale have
been under development since the first whole-genome sequences appeared in the mid …

Costless metabolic secretions as drivers of interspecies interactions in microbial ecosystems

AR Pacheco, M Moel, D Segrè - Nature communications, 2019 - nature.com
Metabolic exchange mediates interactions among microbes, helping explain diversity in
microbial communities. As these interactions often involve a fitness cost, it is unclear how …

A systematic assessment of current genome-scale metabolic reconstruction tools

SN Mendoza, BG Olivier, D Molenaar, B Teusink - Genome biology, 2019 - Springer
Background Several genome-scale metabolic reconstruction software platforms have been
developed and are being continuously updated. These tools have been widely applied to …

The revisited genome of Pseudomonas putida KT2440 enlightens its value as a robust metabolic chassis

E Belda, RGA Van Heck… - Environmental …, 2016 - Wiley Online Library
By the time the complete genome sequence of the soil bacterium Pseudomonas putida
KT2440 was published in 2002 (Nelson et al.,) this bacterium was considered a potential …

Understanding the antimicrobial mechanism of TiO2-based nanocomposite films in a pathogenic bacterium

A Kubacka, MS Diez, D Rojo, R Bargiela, S Ciordia… - Scientific reports, 2014 - nature.com
Abstract Titania (TiO2)-based nanocomposites subjected to light excitation are remarkably
effective in eliciting microbial death. However, the mechanism by which these materials …

High‐quality genome‐scale metabolic modelling of Pseudomonas putida highlights its broad metabolic capabilities

J Nogales, J Mueller, S Gudmundsson… - Environmental …, 2020 - Wiley Online Library
Genome‐scale reconstructions of metabolism are computational species‐specific
knowledge bases able to compute systemic metabolic properties. We present a …

Engineering Native and Synthetic Pathways in Pseudomonas putida for the Production of Tailored Polyhydroxyalkanoates

MP Mezzina, MT Manoli, MA Prieto… - Biotechnology …, 2021 - Wiley Online Library
Growing environmental concern sparks renewed interest in the sustainable production of
(bio) materials that can replace oil‐derived goods. Polyhydroxyalkanoates (PHAs) are …

Reconciled rat and human metabolic networks for comparative toxicogenomics and biomarker predictions

EM Blais, KD Rawls, BV Dougherty, ZI Li… - Nature …, 2017 - nature.com
The laboratory rat has been used as a surrogate to study human biology for more than a
century. Here we present the first genome-scale network reconstruction of Rattus norvegicus …

[HTML][HTML] Biobased PET from lignin using an engineered cis, cis-muconate-producing Pseudomonas putida strain with superior robustness, energy and redox …

M Kohlstedt, A Weimer, F Weiland, J Stolzenberger… - Metabolic …, 2022 - Elsevier
Polyethylene terephthalate (PET), the most common synthetic polyester today, is largely
produced from fossil resources, contributing to global warming. Consequently, sustainable …