Magnetic separations in biotechnology

L Borlido, AM Azevedo, ACA Roque… - Biotechnology …, 2013 - Elsevier
Magnetic separations are probably one of the most versatile separation processes in
biotechnology as they are able to purify cells, viruses, proteins and nucleic acids directly …

Simulating nature in sperm selection for assisted reproduction

ETY Leung, CL Lee, X Tian, KKW Lam, RHW Li… - Nature Reviews …, 2022 - nature.com
Sperm selection in the female reproductive tract (FRT) is sophisticated. Only about 1,000
sperm out of millions in an ejaculate reach the fallopian tube and thus have a chance of …

Sperm selection in assisted reproduction: A review of established methods and cutting-edge possibilities

G Marzano, MS Chiriacò, E Primiceri… - Biotechnology …, 2020 - Elsevier
Male infertility often involves idiopathic or unknown causes, leading to an increasing
demand for assisted reproduction technologies (ART). Conventional sperm sorting …

Sperm selection using magnetic activated cell sorting (MACS) in assisted reproduction: a systematic review and meta-analysis

M Gil, V Sar-Shalom, Y Melendez Sivira… - Journal of assisted …, 2013 - Springer
Purpose To determine whether the use of Magnetic Activated Cell Sorting (MACS) as a
sperm selection technique improves ART success rates in couples undergoing assisted …

Effects of advanced selection methods on sperm quality and ART outcome: a systematic review

TM Said, JA Land - Human reproduction update, 2011 - academic.oup.com
BACKGROUND Current routine semen preparation techniques do not inclusively target all
intrinsic sperm characteristics that may impact the fertilization potential. In order to address …

[HTML][HTML] Sperm preparation: state-of-the-art—physiological aspects and application of advanced sperm preparation methods

R Henkel - Asian journal of andrology, 2012 - ncbi.nlm.nih.gov
For assisted reproduction technologies (ART), numerous techniques were developed to
isolate spermatozoa capable of fertilizing oocytes. While early methodologies only focused …

Not all sperm are equal: functional mitochondria characterize a subpopulation of human sperm with better fertilization potential

AP Sousa, A Amaral, M Baptista, R Tavares… - PloS one, 2011 - journals.plos.org
Human sperm samples are very heterogeneous and include a low amount of truly functional
gametes. Distinct strategies have been developed to characterize and isolate this specific …

Magnetic-activated cell sorting for sperm preparation reduces spermatozoa with apoptotic markers and improves the acrosome reaction in couples with unexplained …

TH Lee, CH Liu, YT Shih, HM Tsao… - Human …, 2010 - academic.oup.com
BACKGROUND Couples with unexplained infertility (UI) tend to have low fertilization rates
with current IVF procedures. Here, we attempted to identify spermatozoa with apoptotic …

Exploration of miRNA and mRNA profiles in fresh and frozen-thawed boar sperm by transcriptome and small RNA sequencing

DH Dai, IH Qazi, MX Ran, K Liang, Y Zhang… - International Journal of …, 2019 - mdpi.com
Due to lower farrowing rate and reduced litter size with frozen-thawed semen, over 90% of
artificial insemination (AI) is conducted using liquid stored boar semen. Although substantial …

Membrane lipid replacement with nano-micelles in human sperm cryopreservation improves post-thaw function and acrosome protein integrity

M Hezavehei, M Sharafi, R Fathi, A Shahverdi… - Reproductive …, 2021 - Elsevier
Research question Membrane lipid replacement (MLR) of oxidized membrane lipids can
restore sperm cellular membrane functionality and help improve surface protein stability …