Achievements and future perspectives of embryo transfer technology in pigs

EA Martinez, CA Martinez, JM Cambra… - … in domestic animals, 2019 - Wiley Online Library
Commercial embryo transfer (ET) has unprecedented productive and economic implications
for the pig sector. However, pig ET has been considered utopian for decades mainly …

Oviductal extracellular vesicles enhance porcine in vitro embryo development by modulating the embryonic transcriptome

AS de Alcântara-Neto, C Cuello, R Uzbekov… - Biomolecules, 2022 - mdpi.com
Oviductal extracellular vesicles (oEVs) have been identified as important components of the
oviductal fluid (OF) and have been pointed to as key modulators of gamete/embryo-maternal …

The role of forskolin as a lipolytic stimulator during in vitro oocyte maturation and the in vitro embryo production of livestock

SHA Raza, AH Abd El‐Aziz… - … in Domestic Animals, 2021 - Wiley Online Library
Cryopreservation is a modern technique which assists in the preservation of genetic material
from oocytes and embryos for a long time. However, elevated vulnerability to …

Cell apoptosis and lipid content of in vitro–produced, vitrified bovine embryos treated with forskolin

DM Paschoal, MJ Sudano, KRL Schwarz… - Theriogenology, 2017 - Elsevier
The presence of fetal calf serum in culture medium influences embryo quality, causing a
reduction in postcryopreservation survival. Forskolin has been used to induce lipolysis and …

Effective vitrification and warming of porcine embryos using a pH-stable, chemically defined medium

C Cuello, CA Martinez, A Nohalez, I Parrilla, J Roca… - Scientific Reports, 2016 - nature.com
The use of pH-stable media would simplify embryo vitrification and the warming of porcine
embryos and might facilitate the application of embryo transfer in practice. In this work, we …

Vitrification, not cryoprotectant exposure, alters the expression of developmentally important genes in in vitro produced porcine blastocysts

LK Bartolac, JL Lowe, G Koustas, CG Grupen… - Cryobiology, 2018 - Elsevier
The vitrification of embryos is common practice in advanced livestock breeding programs
and in human fertility clinics. Recent studies have revealed that vitrification results in …

Unveiling how vitrification affects the porcine blastocyst: clues from a transcriptomic study

C Almiñana, F Dubuisson, S Bauersachs… - Journal of Animal …, 2022 - Springer
Background Currently, there is a high demand for efficient pig embryo cryopreservation
procedures in the porcine industry as well as for genetic diversity preservation and research …

Treatment with cyclic adenosine monophosphate modulators prior to in vitro maturation alters the lipid composition and transcript profile of bovine cumulus–oocyte …

EM Razza, MJ Sudano, PK Fontes… - Reproduction …, 2018 - CSIRO Publishing
Mammalian oocytes resume meiosis spontaneously after removal from the ovarian follicle.
We tested the effects of a 2-h prematuration treatment (Pre-IVM) with forskolin (FSK) and 3 …

Addition of L-ascorbic acid to culture and vitrification media of IVF porcine blastocysts improves survival and reduces HSPA1A levels of vitrified embryos

M Castillo-Martín, M Yeste, A Soler… - Reproduction, Fertility …, 2015 - CSIRO Publishing
The aim of the present study was to determine the effect of l-ascorbic acid on embryo quality
and gene expression of porcine blastocysts after supplementations of in vitro culture …

A comparison of different vitrification devices and the effect of blastocoele collapse on the cryosurvival of in vitro produced porcine embryos

LK Bartolac, JL Lowe, G Koustas… - … of reproduction and …, 2015 - jstage.jst.go.jp
The aim of this study was to determine the optimum conditions for vitrifying in vitro produced
day 7 porcine embryos using different vitrification devices and blastocoele collapse …