Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays

SA Bustin - Journal of molecular endocrinology, 2000 - jme.bioscientifica.com
To investigate whether the tumor suppressor gene PTEN affects the activity of the androgen
receptor (AR), we monitored the expression of the apoptotic gene HA-Bax (inserted in an …

Real-time PCR: a review of approaches to data analysis

DV Rebrikov, DY Trofimov - Applied biochemistry and microbiology, 2006 - Springer
The registration of the accumulation of polymerase chain reaction (PCR) products in the
course of amplification (real-time PCR) requires specific equipment, ie, detecting amplifiers …

15S-hydroxyeicosatetraenoic acid activates peroxisome proliferator-activated receptor γ and inhibits proliferation in PC3 prostate carcinoma cells

SB Shappell, RA Gupta, S Manning, R Whitehead… - Cancer research, 2001 - AACR
Lipoxygenase (15-LOX)-2 is expressed in benign prostate secretory cells and benign
prostate produces 15S-hydroxyeicosatetraenoic acid (15S-HETE) from exogenous …

One carbon metabolism disturbances and the C677T MTHFR gene polymorphism in children with autism spectrum disorders

SP Paşca, E Dronca, T Kaucsár… - Journal of cellular …, 2009 - Wiley Online Library
Autism spectrum disorders (ASDs), which include the prototypic autistic disorder (AD),
Asperger's syndrome (AS) and pervasive developmental disorders not otherwise specified …

CpG DNA induces maturation of dendritic cells with distinct effects on nascent and recycling MHC-II antigen-processing mechanisms

D Askew, RS Chu, AM Krieg… - The Journal of …, 2000 - journals.aai.org
Murine bone marrow cultured with GM-CSF produced dendritic cells (DCs) expressing MHC
class II (MHC-II) but little CD40, CD80, or CD86. Oligodeoxynucleotides (ODN) containing …

Mutation detection using fluorescent hybridization probes and melting curve analysis

E Lyon - Expert review of molecular diagnostics, 2001 - Taylor & Francis
The LightCycler® is a real-time PCR instrument that combines a thermocycler and a micro-
volume fluorimeter. LightCycler technology is gaining popularity due to its ability to detect …

Emerging homogeneous DNA-based technologies in the clinical laboratory

CA Foy, HC Parkes - Clinical Chemistry, 2001 - academic.oup.com
Background: Advances in molecular diagnostic technologies have enabled genetic testing
in single closed-tube reactions. The purpose of this review is to highlight some of the …

Inherited prothrombotic risk factors in children with stroke, transient ischemic attack, or migraine

DC Herak, MR Antolic, JL Krleza, M Pavic, S Dodig… - …, 2009 - publications.aap.org
OBJECTIVE. The aim of this study was to investigate the prevalence and possible
association of inherited prothrombotic risk factors in children with stroke, transient ischemic …

Методы детекции однонуклеотидных полиморфизмов: аллель-специфичная ПЦР и гибридизация с олигонуклеотидной пробой

ИА Кофиади, ДВ Ребриков - Генетика, 2006 - elibrary.ru
Существующее разнообразие методов детекции однонуклеотидных полиморфизмов
настолько велико, что может привести в замешательство неискушенного …

[PDF][PDF] Real-time PCR

SA Bustin - Encyclopedia of diagnostic genomics and …, 2005 - gene-quantification.com
The real-time polymerase chain reaction (PCR) uses fluorescent reporter molecules to
monitor the production of amplification products during each cycle of the PCR reaction. This …