The in vitro production of porcine embryos has presented numerous challenges to
researchers over the past four decades. Some of the problems encountered were specific to …

Genetically modified pigs have become valuable tools for generating advances in animal
agriculture and human medicine. Importantly, in vitro production and manipulation of …

It is now over a decade since the birth, in 1996, of Dolly the first animal to be produced by
nuclear transfer using an adult derived somatic cell as nuclear donor. Since this time similar …

A system for somatic cell nuclear transfer (SCNT) was developed and led to the successful
production of GFP-transfected piglets. In experiment 1, two groups of SCNT couplets …

During the past 6 years, considerable advancement has been achieved in experimental
embryology of pigs. This process was mainly generated by the rapidly increasing need for …

The present study was designed to examine the effect of trichostatin A (TSA), an inhibitor of
histone deacetylase, on development of porcine cloned embryos. Our results showed that …

Selecting oocytes that are most likely to develop is crucial for in vitro fertilization and animal
cloning. Brilliant cresyl blue (BCB) staining has been used for oocyte selection in large …

Over the past decades, in vitro culture media have been developed to successfully support
IVF embryo growth in a variety of species. Advanced reproductive technologies, such as …

Guinea pigs are a valuable animal model for studying various diseases, including
reproductive diseases. However, techniques for generating embryos via embryo …

The objective of this study was to investigate the effects of oocyte selection using brilliant
cresyl blue (BCB) and culture density during individual in vitro maturation (IVM) on porcine …