Chip-compatible wide-field 3D nanoscopy through tunable spatial frequency shift effect

X Liu, M Tang, C Meng, C Pang, C Kuang… - Science China Physics …, 2021 - Springer
Spatial frequency shift (SFS) microscopy with evanescent wave illumination shows intriguing
advantages, including large field of view (FOV), high speed, and good modularity. However …

Multi‐modal adaptor‐clathrin contacts drive coated vesicle assembly

SM Smith, G Larocque, KM Wood, KL Morris… - The EMBO …, 2021 - embopress.org
Clathrin‐coated pits are formed by the recognition of membrane and cargo by the AP2
complex and the subsequent recruitment of clathrin triskelia. A role for AP2 in coated‐pit …

Regulation of acid-sensing ion channels by protein binding partners

MM Cullinan, RC Klipp, JR Bankston - Channels, 2021 - Taylor & Francis
Acid-sensing ion channels (ASICs) are a family of proton-gated cation channels that
contribute to a diverse array of functions including pain sensation, cell death during …

Reconstituting membrane fission using a high content and throughput assay

U Swaminathan, TJ Pucadyil - Biochemical Society Transactions, 2024 - portlandpress.com
Protein-mediated membrane fission has been analyzed both in bulk and at the single event
resolution. Studies on membrane fission in vitro using tethers have provided fundamental …

Confocal interferometric scattering microscopy reveals 3D nanoscopic structure and dynamics in live cells

V Sandoghdar, M Kueppers, D Albrecht, A Kashkanova… - 2022 - researchsquare.com
Bright-field light microscopy and related techniques continue to play a key role in life
sciences because they provide a facile and label-free insight into biological specimen …

[HTML][HTML] Fluidic shear stress alters clathrin dynamics and vesicle formation in endothelial cells

TJ Nawara, E Sztul, AL Mattheyses - bioRxiv, 2024 - ncbi.nlm.nih.gov
Endothelial cells (ECs) experience a variety of highly dynamic mechanical stresses. Among
others, cyclic stretch and increased plasma membrane tension inhibit clathrin-mediated …

Ubiquitin-driven protein condensation initiates clathrin-mediated endocytosis

F Yuan, S Gollapudi, KJ Day, G Ashby, A Sangani… - bioRxiv, 2023 - biorxiv.org
Clathrin-mediated endocytosis is an essential cellular pathway that enables signaling and
recycling of transmembrane proteins and lipids. During endocytosis, dozens of cytosolic …

Diversity, origin, and evolution of the ESCRT systems

KS Makarova, V Tobiasson, YI Wolf, Z Lu, Y Liu… - Mbio, 2024 - Am Soc Microbiol
Endosomal sorting complexes required for transport (ESCRT) play key roles in protein
sorting between membrane-bounded compartments of eukaryotic cells. Homologs of many …

Prodrugs of the Archetypal Dynamin Inhibitor Bis‐T‐22

LR Odell, MJ Robertson, KA Young… - …, 2022 - Wiley Online Library
The Bis‐T series of compounds comprise some of the most potent inhibitors of dynamin
GTPase activity yet reported, eg,(2E, 2′ E)‐N, N′‐(propane‐1, 3‐diyl) bis (2‐cyano‐3‐(3 …

[HTML][HTML] Integrating protein copy numbers with interaction networks to quantify stoichiometry in clathrin-mediated endocytosis

D Duan, M Hanson, DO Holland, ME Johnson - Scientific Reports, 2022 - nature.com
Proteins that drive processes like clathrin-mediated endocytosis (CME) are expressed at
copy numbers within a cell and across cell types varying from hundreds (eg auxilin) to …