To determine which transcripts should reach the cytoplasm for translation, eukaryotic cells
have established mechanisms to regulate selective mRNA export through the nuclear pore …

A common strategy for exploring the biological roles of deubiquitinating enzymes (DUB s) in
different pathways is to study the effects of replacing the wild‐type DUB with a catalytically …

Transcription initiation is a major regulatory step in eukaryotic gene expression. It involves
the assembly of general transcription factors and RNA polymerase II into a functional pre …

The Gcn5 histone acetyltransferase (HAT) subunit of the SAGA transcriptional coactivator
complex catalyzes acetylation of histone H3 and H2B N-terminal tails, posttranslational …

In the last decade, multiple studies demonstrated that cells maintain a balance of mRNA
production and degradation, but the mechanisms by which cells implement this balance …

The transcriptional coactivators Mediator and two histone acetyltransferase (HAT)
complexes, NuA4 and SAGA, play global roles in transcriptional activation. Here we explore …

ABSTRACT A yeast in vitro system was developed that is active for transcription at both
TATA-containing and TATA-less promoters. Transcription with extracts made from cells …

Three classes of yeast protein-coding genes are distinguished by their dependence on the
transcription cofactors TFIID, SAGA, and Mediator (MED) Tail, but whether this dependence …

TFIID is a cornerstone of eukaryotic gene regulation. Distinct TFIID complexes with unique
subunit compositions exist and several TFIID subunits are shared with other complexes …

Gene expression is a highly regulated process that adapts RNAs and proteins content to the
cellular context. Under steady-state conditions, mRNA homeostasis is robustly maintained …