[HTML][HTML] Toward uncharted territory of cellular heterogeneity: advances and applications of single-cell RNA-seq

B Lieberman, M Kusi, CN Hung, CW Chou… - Journal of …, 2021 - ncbi.nlm.nih.gov
Among single-cell analysis technologies, single-cell RNA-seq (scRNA-seq) has been one of
the front runners in technical inventions. Since its induction, scRNA-seq has been well …

Isocell: An approach to enhance single cell clustering by integrating isoform-level expression through orthogonal projection

Y Liu, HD Li, Y Xu, YW Liu, X Peng… - IEEE/ACM Transactions …, 2022 - ieeexplore.ieee.org
Single cell RNA sequencing (scRNA-seq) provides a powerful approach for profiling
transcriptomes at single cell resolution. An essential application of scRNA-seq is the …

[HTML][HTML] A flexible microfluidic system for single-cell transcriptome profiling elucidates phased transcriptional regulators of cell cycle

K Davey, D Wong, F Konopacki, E Kwa, T Ly… - Scientific Reports, 2021 - nature.com
Single cell transcriptome profiling has emerged as a breakthrough technology for the high-
resolution understanding of complex cellular systems. Here we report a flexible, cost …

[HTML][HTML] Exploring the complexity of cortical development using single-cell transcriptomics

H Jeong, VK Tiwari - Frontiers in Neuroscience, 2018 - frontiersin.org
The developing neocortex in the mammalian brain is composed of multiple cell types
including apical progenitors (AP), basal progenitors (BP), and neurons that populate three …

Single‐cell RNA‐seq: Introduction to bioinformatics analysis

F Ji, RI Sadreyev - Current protocols in molecular biology, 2019 - Wiley Online Library
Quantitative analysis of single‐cell RNA sequencing (RNA‐seq) is crucial for discovering the
heterogeneity of cell populations and understanding the molecular mechanisms in different …

Exploiting single-cell RNA sequencing data to link alternative splicing and cancer heterogeneity: A computational approach

I Manipur, I Granata, MR Guarracino - The International Journal of …, 2019 - Elsevier
Cell heterogeneity studies using single-cell sequencing are gaining great significance in the
era of personalized medicine. In particular, characterization of tumor heterogeneity is an …

Single-cell analysis of the transcriptome and epigenome

K Mazan-Mamczarz, J Ha, S De, P Sen - Computational Systems Biology …, 2022 - Springer
Epigenome regulation has emerged as an important mechanism for the maintenance of
organ function in health and disease. Dissecting epigenomic alterations and resultant gene …

Uncertainty versus variability: Bayesian methods for analysis of scRNA-seq data

Y Huang, G Sanguinetti - Current Opinion in Systems Biology, 2021 - Elsevier
Single-cell 'omics technologies have the potential to revolutionize our understanding of
stochasticity and heterogeneity in biology, yet such measurements are inevitably affected by …

[HTML][HTML] A scalable strand-specific protocol enabling full-length total RNA sequencing from single cells

S Haile, RD Corbett, VG LeBlanc, L Wei… - Frontiers in …, 2021 - frontiersin.org
RNA sequencing (RNAseq) has been widely used to generate bulk gene expression
measurements collected from pools of cells. Only relatively recently have single-cell …

An NMF-based approach to discover overlooked differentially expressed gene regions from single-cell RNA-seq data

H Matsumoto, T Hayashi, H Ozaki… - NAR Genomics and …, 2020 - academic.oup.com
Single-cell RNA sequencing has enabled researchers to quantify the transcriptomes of
individual cells, infer cell types and investigate differential expression among cell types …