Reference genes in real-time PCR

B Kozera, M Rapacz - Journal of applied genetics, 2013 - Springer
This paper aims to discuss various aspects of the use of reference genes in qPCR technique
used in the thousands of present studies. Most frequently, these are housekeeping genes …

Introduction to the gene expression analysis

I San Segundo-Val, CS Sanz-Lozano - Molecular genetics of asthma, 2016 - Springer
Abstract In 1941, Beadle and Tatum published experiments that would explain the basis of
the central dogma of molecular biology, whereby the DNA through an intermediate …

Transgenic banana plants overexpressing a native plasma membrane aquaporin MusaPIP1;2 display high tolerance levels to different abiotic stresses

S Sreedharan, UKS Shekhawat… - Plant Biotechnology …, 2013 - Wiley Online Library
Water transport across cellular membranes is regulated by a family of water channel
proteins known as aquaporins (AQP s). As most abiotic stresses like suboptimal …

Comprehensive selection of reference genes for gene expression normalization in sugarcane by real time quantitative RT-PCR

H Ling, Q Wu, J Guo, L Xu, Y Que - PloS one, 2014 - journals.plos.org
The increasingly used real time quantitative reverse transcription-PCR (qRT-PCR) method
for gene expression analysis requires one or several reference gene (s) acting as …

From crossbreeding to biotechnology-facilitated improvement of banana and plantain

R Ortiz, R Swennen - Biotechnology Advances, 2014 - Elsevier
The annual harvest of banana and plantain (Musa spp.) is approximately 145 million tons
worldwide. About 85% of this global production comes from small plots and kitchen or …

Banana NAC transcription factor MusaNAC042 is positively associated with drought and salinity tolerance

H Tak, S Negi, TR Ganapathi - Protoplasma, 2017 - Springer
Banana is an important fruit crop and its yield is hampered by multiple abiotic stress
conditions encountered during its growth. The NAC (NAM, ATAF, and CUC) transcription …

MusaSAP1, a A20/AN1 zinc finger gene from banana functions as a positive regulator in different stress responses

S Sreedharan, UKS Shekhawat, TR Ganapathi - Plant molecular biology, 2012 - Springer
A20/AN1 zinc finger domain containing Stress Associated Proteins (SAP) are involved in
diverse stress response pathways in plants. In the present study, a novel banana SAP gene …

Identification and Validation of Reference Genes for Transcript Normalization in Strawberry (Fragaria × ananassa) Defense Responses

F Amil-Ruiz, J Garrido-Gala, R Blanco-Portales… - PloS one, 2013 - journals.plos.org
Strawberry (Fragaria spp) is an emerging model for the development of basic genomics and
recombinant DNA studies among rosaceous crops. Functional genomic and molecular …

A banana NAC transcription factor (MusaSNAC1) impart drought tolerance by modulating stomatal closure and H2O2 content

S Negi, H Tak, TR Ganapathi - Plant molecular biology, 2018 - Springer
Key message MusaSNAC1 function in H 2 O 2 mediated stomatal closure and promote
drought tolerance by directly binding to CGT [A/G] motif in regulatory region of multiple stress …

Constitutive and stress-inducible overexpression of a native aquaporin gene (MusaPIP2;6) in transgenic banana plants signals its pivotal role in salt tolerance

S Sreedharan, UKS Shekhawat, TR Ganapathi - Plant Molecular Biology, 2015 - Springer
High soil salinity constitutes a major abiotic stress and an important limiting factor in
cultivation of crop plants worldwide. Here, we report the identification and characterization of …