Mechanistic insights into HuR inhibitor MS-444 arresting embryonic development revealed by low-input RNA-seq and STORM

Y Nie, W Xu, GG Tian, X Li, Y Guo, X Liu, L He… - Cell Biology and …, 2022 - Springer
With improvements in the survival rate of patients with cancer, fertility maintenance has
become a major concern in terms of cancer treatment for women of reproductive age. Thus …

Cooperativity boosts affinity and specificity of proteins with multiple RNA-binding domains

SH Stitzinger, S Sohrabi-Jahromi… - NAR Genomics and …, 2023 - academic.oup.com
Numerous cellular processes rely on the binding of proteins with high affinity to specific sets
of RNAs. Yet most RNA-binding domains display low specificity and affinity in comparison to …

Episomes and Transposases—Utilities to Maintain Transgene Expression from Nonviral Vectors

F Kreppel, C Hagedorn - Genes, 2022 - mdpi.com
The efficient delivery and stable transgene expression are critical for applications in gene
therapy. While carefully selected and engineered viral vectors allowed for remarkable …

Why to target G‐quadruplexes using peptides: Next‐generation G4‐interacting ligands

T Sharma, N Kundu, S Kaur… - Journal of Peptide …, 2023 - Wiley Online Library
Guanine‐rich oligonucleotides existing in both DNA and RNA are able to fold into four‐
stranded DNA secondary structures via Hoogsteen type hydrogen‐bonding, where four …

[HTML][HTML] Ribonucleotide reductase small subunit M2 promotes the proliferation of esophageal squamous cell carcinoma cells via HuR-mediated mRNA stabilization

J Zhang, Q Wu, Y Xie, F Li, H Wei, Y Jiang… - … Pharmaceutica Sinica B, 2024 - Elsevier
Esophageal squamous cell carcinoma (ESCC), a malignancy of the digestive system, is
highly prevalent and the primary cause of cancer-related deaths worldwide due to the lack of …

The solution structure of Dead End bound to AU-rich RNA reveals an unprecedented mode of tandem RRM-RNA recognition required for mRNA regulation

MM Duszczyk, H Wischnewski, T Kazeeva, FE Loughlin… - BioRxiv, 2019 - biorxiv.org
Dead End (DND1) is an RNA-binding protein essential for germline development through its
role in post-transcriptional gene regulation. The molecular mechanisms behind selection …

The mRNA‐Binding Protein HuR Is a Kinetically‐Privileged Electrophile Sensor

JR Poganik, AK Van Hall‐Beauvais… - Helvetica chimica …, 2020 - Wiley Online Library
The key mRNA‐binding proteins HuR and AUF1 are reported stress sensors in mammals.
Intrigued by recent reports of sensitivity of these proteins to the electrophilic lipid …

[HTML][HTML] CXCL4 is a driver of cytokine mRNA stability in monocyte-derived dendritic cells

SC Silva-Cardoso, CPJ Bekker, M Boes… - Molecular …, 2019 - Elsevier
The chemokine CXCL4 has been implicated in several immune diseases. Exposure of
monocyte-derived dendritic cells (moDCs) to CXCL4 potentiates the production of …

Label-free horizontal EMSA for analysis of protein-RNA interactions

W Perea, NL Greenbaum - Analytical biochemistry, 2020 - Elsevier
We describe a method to analyze the affinity and specificity of interactions between proteins
and RNA using horizontal PAGE under non-denaturing conditions. The method permits …

The Drosophila RNA binding protein Hrp48 binds a specific RNA sequence of the msl-2 mRNA 3'UTR to regulate translation

A Lomoschitz, J Meyer, T Guitart, M Krepl, K Lapouge… - bioRxiv, 2024 - biorxiv.org
Repression of msl-2 mRNA translation is essential for viability of Drosophila melanogaster
females to prevent hypertranscription of both X chromosomes. This translational control …