Over the past decade, new methods and procedures have been developed to generate
genetically engineered mouse models of human disease. This At a Glance article highlights …

Background Conditional knockout mice and transgenic mice expressing recombinases,
reporters, and inducible transcriptional activators are key for many genetic studies and …

Porcine Reproductive and Respiratory Syndrome (PRRS) is a panzootic infectious disease
of pigs, causing major economic losses to the world-wide pig industry. PRRS manifests …

The generation of gene-edited animals using the CRISPRs/Cas9 system is based on
microinjection into zygotes which is inefficient, time consuming and demands high technical …

Two technologies, in vitro culture and exogenous gene introduction, constitute cornerstones
of producing transgenic animals. Although in vitro embryo production techniques can …

Regeneration-capable flatworms are informative research models to study the mechanisms
of stem cell regulation, regeneration, and tissue patterning. However, the lack of …

Objective Cytoplasmic microinjection and electroporation of the CRISPR/Cas9 system into
zygotes are used for generating genetically modified pigs. However, these methods create …

The domestic ferret (Mustela putorius furo) has proven to be a useful species for modeling
human genetic and infectious diseases of the lung and brain. However, biomedical research …

Recently, genome editing in mouse zygotes has become convenient and scalable, in
association with various technological developments and improvements such as novel …

Materials and Methods: We introduced guide RNAs targeting PDX1, GGTA1, and CMAH
with the Cas9 protein into IVF zygotes (one-cell stage) through EP 10 h after the start of IVF …