Fluorescence arguably constitutes the most widely used experimental approach in the field
of protein folding. Hence, any review of the use of fluorescence to probe protein stability and …

In biological microscopy, the ever expanding range of applications requires quantitative
approaches that analyze several distinct fluorescent molecules at the same time in the same …

Optical approaches for observing the dynamics of single molecules have required pico-to
nanomolar concentrations of fluorophore in order to isolate individual molecules. However …

The yeast prion protein Sup35 is a translation termination factor, whose activity is modulated
by sequestration into a self-perpetuating amyloid. The prion-determining domain, NM …

The elegance of animal body plans derives from an intimate connection between function
and form, which during organ formation is linked to patterning and growth. Yet, how …

▪ Abstract We review recent developments in single-molecule spectroscopy and microscopy.
New optical methods provide access to the absorption, emission, or excitation spectra of …

Biogenic amines are organic polycations derived from aromatic or cationic amino acids. All
of them have one or more positive charges and a hydrophobic skeleton. Nature has evolved …

Publisher Summary This chapter discusses microscope technologies for imaging living
fungal cells at high spatial resolution and reviews the vital fluorescent dyes that are proving …

Elucidating protein functions and molecular organisation requires to localise precisely single
or aggregated molecules and analyse their spatial distributions. We develop a statistical …

The controlled addition of structurally defined components to live cell membranes can
facilitate the molecular level analysis of cell surface phenomena. Here we demonstrate that …