Genome editing technology has rapidly evolved to knock-out genes, create targeted genetic
variation, install precise insertion/deletion and single nucleotide changes, and perform large …

Precise replacement of an allele with an elite allele controlling an important agronomic trait
in a predefined manner by gene editing technologies is highly desirable in crop …

The elucidation of protein function and its exploitation in bioengineering have greatly
advanced the life sciences. Protein mining efforts generally rely on amino acid sequences …

CRISPR-Cas9, its derived base editors and CRISPR activation systems have greatly aided
genome engineering in plants. However, these systems are mostly used separately, leaving …

Prime editing is limited by low efficiency in plants. Here, we develop an upgraded
engineered plant prime editor in hexaploid wheat, ePPEplus, by introducing a V223A …

Alongside the use of fertilizer and chemical control of weeds, pests, and diseases modern
breeding has been very successful in generating cultivars that have increased agricultural …

The global climate change and unfavourable abiotic and biotic factors are limiting
agricultural productivity and therefore intensifying the challenges for crop scientists to meet …

A subset of plant intracellular NLR immune receptors detect effector proteins, secreted by
phytopathogens to promote infection, through unconventional integrated domains which …

CRISPR/Cas9 gene technology is transported as RNA from transgenic roots to distal parts of
unmodified grafted scion, where it is translated into proteins to induce heritable mutagenesis …

Cas12f is a hypercompact type V, Cas12 family member. Previously, we reported a set of
engineered guide RNAs supporting high indel efficiency for Cas12f1 in human cells. Here …