Image sensors capable of capturing individual photons have made tremendous progress in
recent years. However, this technology faces a major limitation. Because they capture scene …

Here we use a combination of two-photon Fluorescence Lifetime Imaging Microscopy (FLIM)
of NAD (P) H free/bound ratio in living HIs with post-fixation, immunofluorescence-based …

In the field of fluorescence microscopy, there is continued demand for dynamic technologies
that can exploit the complete information from every pixel of an image. One imaging …

Automated computational analysis techniques utilizing machine learning have been
demonstrated to be able to extract more data from different imaging modalities compared to …

Conventional histopathology involves expensive and labor-intensive processes that often
consume tissue samples, rendering them unavailable for other analyses. We present a …

Histopathology is the foundation and gold standard for identifying diseases, and precise
quantification of histopathological images can provide the pathologist with objective clues to …

NADH and NADPH play key roles in the regulation of metabolism. Their endogenous
fluorescence is sensitive to enzyme binding, allowing changes in cellular metabolic state to …

Endogenous NAD (P) H and FAD two-photon excited fluorescence (TPEF) images provide
functional metabolic information with high spatial resolution for a wide range of living …

Fluorescence lifetime imaging microscopy (FLIM) evaluates the metabolic state of tissue
based on reduced nicotinamide adenine dinucleotide (NAD (P) H) and flavin adenine …

Significance: A major obstacle to studying resident microglia has been their similarity to
infiltrating immune cell types and the lack of unique protein markers for identifying the …