A pseudovirus system enables deep mutational scanning of the full SARS-CoV-2 spike
B Dadonaite, KHD Crawford, CE Radford, AG Farrell… - Cell, 2023 - cell.com
A major challenge in understanding SARS-CoV-2 evolution is interpreting the antigenic and
functional effects of emerging mutations in the viral spike protein. Here, we describe a deep …
functional effects of emerging mutations in the viral spike protein. Here, we describe a deep …
Deep mutational scans of XBB. 1.5 and BQ. 1.1 reveal ongoing epistatic drift during SARS-CoV-2 evolution
AL Taylor, TN Starr - PLoS Pathogens, 2023 - journals.plos.org
Substitutions that fix between SARS-CoV-2 variants can transform the mutational landscape
of future evolution via epistasis. For example, large epistatic shifts in mutational effects …
of future evolution via epistasis. For example, large epistatic shifts in mutational effects …
Advancing antibody engineering through synthetic evolution and machine learning
Abs are versatile molecules with the potential to achieve exceptional binding to target Ags,
while also possessing biophysical properties suitable for therapeutic drug development …
while also possessing biophysical properties suitable for therapeutic drug development …
Deep mutational scanning of proteins in mammalian cells
S Maes, N Deploey, F Peelman, S Eyckerman - Cell Reports Methods, 2023 - cell.com
Protein mutagenesis is essential for unveiling the molecular mechanisms underlying protein
function in health, disease, and evolution. In the past decade, deep mutational scanning …
function in health, disease, and evolution. In the past decade, deep mutational scanning …
High-throughput identification of prefusion-stabilizing mutations in SARS-CoV-2 spike
Designing prefusion-stabilized SARS-CoV-2 spike is critical for the effectiveness of COVID-
19 vaccines. All COVID-19 vaccines in the US encode spike with K986P/V987P mutations to …
19 vaccines. All COVID-19 vaccines in the US encode spike with K986P/V987P mutations to …
Deep mutagenesis scanning using whole trimeric SARS-CoV-2 spike highlights the importance of NTD-RBD interactions in determining spike phenotype
R Kugathasan, K Sukhova, M Moshe, P Kellam… - PLoS …, 2023 - journals.plos.org
New variants of SARS-CoV-2 are continually emerging with mutations in spike associated
with increased transmissibility and immune escape. Phenotypic maps can inform the …
with increased transmissibility and immune escape. Phenotypic maps can inform the …
SARS-CoV-2 resistance to monoclonal antibodies and small-molecule drugs
S Iketani, DD Ho - Cell Chemical Biology, 2024 - cell.com
Over four years have passed since the beginning of the COVID-19 pandemic. The scientific
response has been rapid and effective, with many therapeutic monoclonal antibodies and …
response has been rapid and effective, with many therapeutic monoclonal antibodies and …
Functional and antigenic characterization of SARS-CoV-2 spike fusion peptide by deep mutational scanning
The fusion peptide of SARS-CoV-2 spike protein is functionally important for membrane
fusion during virus entry and is part of a broadly neutralizing epitope. However, sequence …
fusion during virus entry and is part of a broadly neutralizing epitope. However, sequence …
High-throughput screening of mutations affecting SARS-CoV-2 spike functions
S Xia, Z Liu, S Jiang - Trends in Immunology, 2023 - cell.com
The spike (S) protein of SARS-CoV-2, which is undergoing rapid evolution, plays crucial
roles in viral immune escape, infectivity, and transmissibility. To gain clinical insight …
roles in viral immune escape, infectivity, and transmissibility. To gain clinical insight …
High-throughput screening of spike variants uncovers the key residues that alter the affinity and antigenicity of SARS-CoV-2
Y Luo, S Liu, J Xue, Y Yang, J Zhao, Y Sun, B Wang… - Cell Discovery, 2023 - nature.com
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection has elicited a
worldwide pandemic since late 2019. There has been~ 675 million confirmed coronavirus …
worldwide pandemic since late 2019. There has been~ 675 million confirmed coronavirus …