The application of sequencing technology is shifting from research to clinical laboratories
owing to rapid technological developments and substantially reduced costs. However …

Background Metagenomic next-generation sequencing (mNGS) has changed the diagnosis
landscape of lower respiratory tract infections (LRIs). With the development of newer …

Lower respiratory tract infections are associated with high morbidity and mortality and
significant clinical harm. Due to the limited ability of traditional pathogen detection methods …

Metagenomic next-generation sequencing (mNGS) of microbial cell-free DNA (mcfDNA
sequencing) is becoming an attractive diagnostic modality for infectious diseases, allowing …

Background A prosthetic joint infection (PJI) is a devastating complication following total joint
arthroplasties with poor prognosis. Identifying an accurate and prompt diagnostic method is …

Bacterial pathogens are one of the major threats to biosafety and environmental health, and
advanced assessment is a prerequisite to combating bacterial pathogens. Currently, 16S …

Objective: To evaluate the diagnostic performance of metagenomic next-generation
sequencing (mNGS) using bronchoalveolar lavage fluid (BALF) in patients with ventilator …

Environmental pollution is escalating due to rapid global development that often prioritizes
human needs over planetary health. Despite global efforts to mitigate legacy pollutants, the …

Increasing globalization, agricultural intensification, urbanization, and climatic changes have
resulted in a significant recent increase in emerging infectious zoonotic diseases. Zoonotic …

Objectives This study aimed to evaluate the clinical value of metagenomic next-generation
sequencing (mNGS) in patients with suspected lower respiratory tract infections. Methods …