The CRISPR-Cas toolbox and gene editing technologies
G Liu, Q Lin, S Jin, C Gao - Molecular cell, 2022 - cell.com
The emergence of CRISPR-Cas systems has accelerated the development of gene editing
technologies, which are widely used in the life sciences. To improve the performance of …
technologies, which are widely used in the life sciences. To improve the performance of …
Advances in nucleic acid amplification techniques (NAATs): COVID-19 point-of-care diagnostics as an example
Achieving superhigh sensitivity is the ultimate goal for bio-detection in modern analytical
science and life science. Among variable signal amplification strategies, nucleic acid …
science and life science. Among variable signal amplification strategies, nucleic acid …
[HTML][HTML] Multiplexed CRISPR-based microfluidic platform for clinical testing of respiratory viruses and identification of SARS-CoV-2 variants
Abstract The coronavirus disease 2019 (COVID-19) pandemic has demonstrated a clear
need for high-throughput, multiplexed and sensitive assays for detecting severe acute …
need for high-throughput, multiplexed and sensitive assays for detecting severe acute …
Characterization of a thermostable Cas13 enzyme for one-pot detection of SARS-CoV-2
Type VI CRISPR-Cas systems have been repurposed for various applications such as gene
knockdown, viral interference, and diagnostics. However, the identification and …
knockdown, viral interference, and diagnostics. However, the identification and …
A smartphone-based visual biosensor for CRISPR-Cas powered SARS-CoV-2 diagnostics
L Ma, L Yin, X Li, S Chen, L Peng, G Liu, S Ye… - Biosensors and …, 2022 - Elsevier
The pandemic of coronavirus disease 2019 (COVID-19) resulted from novel severe acute
respiratory syndrome coronavirus 2 (SARS-CoV-2) has become a worldwide concern. It is …
respiratory syndrome coronavirus 2 (SARS-CoV-2) has become a worldwide concern. It is …
SERS-based CRISPR/Cas assay on microfluidic paper analytical devices for supersensitive detection of pathogenic bacteria in foods
J Zhuang, Z Zhao, K Lian, L Yin, J Wang, S Man… - Biosensors and …, 2022 - Elsevier
Rapid and point-of-need (PON) detection of bacteria is crucial to directly provide rapid and
reliable diagnostics information during on-site tests, allowing more room for taking proactive …
reliable diagnostics information during on-site tests, allowing more room for taking proactive …
Novel non-nucleic acid targets detection strategies based on CRISPR/Cas toolboxes: A review
X Cheng, Y Li, J Kou, D Liao, W Zhang, L Yin… - Biosensors and …, 2022 - Elsevier
Various non-nucleic acid targets (ions, small molecules, polysaccharides, peptides/proteins/
enzymes, cells, transcription factors) are important biomarkers. They play important roles in …
enzymes, cells, transcription factors) are important biomarkers. They play important roles in …
Nanozyme-catalysed CRISPR assay for preamplification-free detection of non-coding RNAs
CRISPR-based diagnostics enable specific sensing of DNA and RNA biomarkers
associated with human diseases. This is achieved through the binding of guide RNAs to a …
associated with human diseases. This is achieved through the binding of guide RNAs to a …
Two years into the COVID-19 pandemic: lessons learned
SJR da Silva, JCF do Nascimento… - ACS infectious …, 2022 - ACS Publications
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a highly transmissible
and virulent human-infecting coronavirus that emerged in late December 2019 in Wuhan …
and virulent human-infecting coronavirus that emerged in late December 2019 in Wuhan …
Photocontrolled crRNA activation enables robust CRISPR-Cas12a diagnostics
M Hu, Z Qiu, Z Bi, T Tian, Y Jiang… - Proceedings of the …, 2022 - National Acad Sciences
CRISPR diagnostics based on nucleic acid amplification faces barriers to its commercial
use, such as contamination risks and insufficient sensitivity. Here, we propose a robust …
use, such as contamination risks and insufficient sensitivity. Here, we propose a robust …