Challenges Associated With the Formation of Recombinant Protein Inclusion Bodies in Escherichia coli and Strategies to Address Them for Industrial Applications

A Bhatwa, W Wang, YI Hassan, N Abraham… - … in Bioengineering and …, 2021 - frontiersin.org
Recombinant proteins are becoming increasingly important for industrial applications, where
Escherichia coli is the most widely used bacterial host for their production. However, the …

Evolution of Escherichia coli Expression System in Producing Antibody Recombinant Fragments

A Sandomenico, JP Sivaccumar, M Ruvo - International Journal of …, 2020 - mdpi.com
Antibodies and antibody-derived molecules are continuously developed as both therapeutic
agents and key reagents for advanced diagnostic investigations. Their application in these …

Current state of [FeFe]-hydrogenase research: biodiversity and spectroscopic investigations

H Land, M Senger, G Berggren, ST Stripp - ACS catalysis, 2020 - ACS Publications
Hydrogenases are redox enzymes that catalyze the conversion of protons and molecular
hydrogen (H2). Based on the composition of the active site cofactor, the monometallic [Fe] …

Plant-produced receptor-binding domain of SARS-CoV-2 elicits potent neutralizing responses in mice and non-human primates

K Siriwattananon, S Manopwisedjaroen… - Frontiers in plant …, 2021 - frontiersin.org
The emergence of coronavirus disease 2019 (COVID-19) caused by severe acute
respiratory syndrome coronavirus 2 (SARS-CoV-2) has affected global public health and …

Factors involved in heterologous expression of proteins in E. coli host

M Pouresmaeil, S Azizi-Dargahlou - Archives of Microbiology, 2023 - Springer
The production of recombinant proteins is one of the most significant achievements of
biotechnology in the last century. These proteins are produced in the eukaryotic or …

Critical review of plant cell wall matrix polysaccharide glycosyltransferase activities verified by heterologous protein expression

RA Amos, D Mohnen - Frontiers in plant science, 2019 - frontiersin.org
The life cycle and development of plants requires the biosynthesis, deposition, and
degradation of cell wall matrix polysaccharides. The structures of the diverse cell wall matrix …

Structural insights into the design of synthetic nanobody libraries

MS Valdés-Tresanco, A Molina-Zapata, AG Pose… - Molecules, 2022 - mdpi.com
Single domain antibodies from camelids, or nanobodies, are a unique class of antibody
fragments with several advantageous characteristics: small monomeric size, high stability …

Single‐chain fragment variable produced by phage display technology: Construction, selection, mutation, expression, and recent applications in food safety

L Li, S Wu, Y Si, H Li, X Yin… - Comprehensive Reviews in …, 2022 - Wiley Online Library
Immunoassays are reliable, efficient, and accurate methods for the analysis of small‐
molecule harmful substances (such as pesticides, veterinary drugs, and biological toxins) …

[HTML][HTML] Growth-coupled enzyme engineering through manipulation of redox cofactor regeneration

JR Nielsen, RA Weusthuis, WE Huang - Biotechnology Advances, 2023 - Elsevier
Enzymes need to be efficient, robust, and highly specific for their effective use in commercial
bioproduction. These properties can be introduced using various enzyme engineering …

Screening of DNA-encoded small molecule libraries inside a living cell

LK Petersen, AB Christensen, J Andersen… - Journal of the …, 2021 - ACS Publications
DNA-encoded small molecule libraries (DELs) have facilitated the discovery of novel
modulators of many different therapeutic protein targets. We report the first successful …