Challenges Associated With the Formation of Recombinant Protein Inclusion Bodies in Escherichia coli and Strategies to Address Them for Industrial Applications
A Bhatwa, W Wang, YI Hassan, N Abraham… - … in Bioengineering and …, 2021 - frontiersin.org
Recombinant proteins are becoming increasingly important for industrial applications, where
Escherichia coli is the most widely used bacterial host for their production. However, the …
Escherichia coli is the most widely used bacterial host for their production. However, the …
Evolution of Escherichia coli Expression System in Producing Antibody Recombinant Fragments
A Sandomenico, JP Sivaccumar, M Ruvo - International Journal of …, 2020 - mdpi.com
Antibodies and antibody-derived molecules are continuously developed as both therapeutic
agents and key reagents for advanced diagnostic investigations. Their application in these …
agents and key reagents for advanced diagnostic investigations. Their application in these …
Current state of [FeFe]-hydrogenase research: biodiversity and spectroscopic investigations
Hydrogenases are redox enzymes that catalyze the conversion of protons and molecular
hydrogen (H2). Based on the composition of the active site cofactor, the monometallic [Fe] …
hydrogen (H2). Based on the composition of the active site cofactor, the monometallic [Fe] …
Plant-produced receptor-binding domain of SARS-CoV-2 elicits potent neutralizing responses in mice and non-human primates
K Siriwattananon, S Manopwisedjaroen… - Frontiers in plant …, 2021 - frontiersin.org
The emergence of coronavirus disease 2019 (COVID-19) caused by severe acute
respiratory syndrome coronavirus 2 (SARS-CoV-2) has affected global public health and …
respiratory syndrome coronavirus 2 (SARS-CoV-2) has affected global public health and …
Factors involved in heterologous expression of proteins in E. coli host
M Pouresmaeil, S Azizi-Dargahlou - Archives of Microbiology, 2023 - Springer
The production of recombinant proteins is one of the most significant achievements of
biotechnology in the last century. These proteins are produced in the eukaryotic or …
biotechnology in the last century. These proteins are produced in the eukaryotic or …
Critical review of plant cell wall matrix polysaccharide glycosyltransferase activities verified by heterologous protein expression
RA Amos, D Mohnen - Frontiers in plant science, 2019 - frontiersin.org
The life cycle and development of plants requires the biosynthesis, deposition, and
degradation of cell wall matrix polysaccharides. The structures of the diverse cell wall matrix …
degradation of cell wall matrix polysaccharides. The structures of the diverse cell wall matrix …
Structural insights into the design of synthetic nanobody libraries
MS Valdés-Tresanco, A Molina-Zapata, AG Pose… - Molecules, 2022 - mdpi.com
Single domain antibodies from camelids, or nanobodies, are a unique class of antibody
fragments with several advantageous characteristics: small monomeric size, high stability …
fragments with several advantageous characteristics: small monomeric size, high stability …
Single‐chain fragment variable produced by phage display technology: Construction, selection, mutation, expression, and recent applications in food safety
L Li, S Wu, Y Si, H Li, X Yin… - Comprehensive Reviews in …, 2022 - Wiley Online Library
Immunoassays are reliable, efficient, and accurate methods for the analysis of small‐
molecule harmful substances (such as pesticides, veterinary drugs, and biological toxins) …
molecule harmful substances (such as pesticides, veterinary drugs, and biological toxins) …
[HTML][HTML] Growth-coupled enzyme engineering through manipulation of redox cofactor regeneration
Enzymes need to be efficient, robust, and highly specific for their effective use in commercial
bioproduction. These properties can be introduced using various enzyme engineering …
bioproduction. These properties can be introduced using various enzyme engineering …
Screening of DNA-encoded small molecule libraries inside a living cell
LK Petersen, AB Christensen, J Andersen… - Journal of the …, 2021 - ACS Publications
DNA-encoded small molecule libraries (DELs) have facilitated the discovery of novel
modulators of many different therapeutic protein targets. We report the first successful …
modulators of many different therapeutic protein targets. We report the first successful …