Metabolic Engineering of Bacterial Respiration: High vs. Low P/O and the Case of Zymomonas mobilis

U Kalnenieks, E Balodite, R Rutkis - Frontiers in Bioengineering and …, 2019 - frontiersin.org
Respiratory chain plays a pivotal role in the energy and redox balance of aerobic bacteria.
By engineering respiration, it is possible to alter the efficiency of energy generation and …

Elimination of editing plasmid mediated by theophylline riboswitch in Zymomonas mobilis

Y Huang, M Chen, G Hu, B Wu, M He - Applied Microbiology and …, 2023 - Springer
Zymomonas mobilis is regarded as a potential chassis for the production of platform
chemicals. Genome editing using the CRISPR-Cas system could meet the need for gene …

Conversion of cellulose and hemicellulose of biomass simultaneously to acetoin by thermophilic simultaneous saccharification and fermentation

X Jia, X Peng, Y Liu, Y Han - Biotechnology for biofuels, 2017 - Springer
Abstract Background Acetoin (3-hydroxy-2-butanone), the precursor of biofuel 2, 3-
butanediol, is an important bio-based platform chemical with wide applications. Fermenting …

CRISPR-mediated host genomic DNA damage is efficiently repaired through microhomology-mediated end joining in Zymomonas mobilis

X Wang, B Wu, X Sui, Z Zhang, T Liu, Y Li, G Hu… - Journal of Genetics and …, 2021 - Elsevier
CRISPR-Cas systems provide bacteria and archaea with adaptive immunity against mobile
genetic elements (MGEs) through uptake of invader-derived spacers. De novo adaptation …

Double nicking by RNA-directed Cascade-nCas3 for high-efficiency large-scale genome engineering

Y Hao, Q Wang, J Li, S Yang, Y Zheng… - Open …, 2022 - royalsocietypublishing.org
New CRISPR-based genome editing technologies are developed to continually drive
advances in life sciences, which, however, are predominantly derived from systems of Type …

Shake flask methodology for assessing the influence of the maximum oxygen transfer capacity on 2, 3-butanediol production

B Heyman, R Lamm, H Tulke, L Regestein… - Microbial Cell …, 2019 - Springer
Abstract Background Production of 2, 3-butanediol from renewable resources is a promising
measure to decrease the consumption of fossil resources in the chemical industry. One of …

Metabolic engineering of Parageobacillus thermoglucosidasius for the efficient production of (2R, 3R)-butanediol

J Zhou, J Lian, CV Rao - Applied microbiology and biotechnology, 2020 - Springer
High-temperature fermentation using thermophilic microorganisms may provide cost-
effective processes for the industrial production of fuels and chemicals, due to decreased …

Effect of pelleting on the enzymatic digestibility of corn stover

X Chen, ACF Dos Santos, DMR Gutierrez, P Song… - Bioresource …, 2023 - Elsevier
Pelleting of lignocellulosic biomass to improve its transportation, storage and handling
impacts subsequent processing and conversion. This work reports the role of high moisture …

The Magnesium Concentration in Yeast Extracts Is a Major Determinant Affecting Ethanol Fermentation Performance of Zymomonas mobilis

R Li, M Jin, J Du, M Li, S Chen, S Yang - Frontiers in Bioengineering …, 2020 - frontiersin.org
Zymomonas mobilis is a model ethanologenic bacterium for diverse biochemical production.
Rich medium (RM) is a complex medium that is routinely used to cultivate Z. mobilis, which …

Determination of Nucleotide Sequences within Promoter Regions Affecting Promoter Compatibility between Zymomonas mobilis and Escherichia coli

H Song, Y Yang, H Li, J Du, Z Hu, Y Chen… - ACS Synthetic …, 2022 - ACS Publications
A promoter plays a crucial role in controlling the expression of the target gene in cells, thus
being one of the key biological parts for synthetic biology practices. Although significant …