Endogenous CRISPR-assisted microhomology-mediated end joining enables rapid genome editing in Zymomonas mobilis

X Sui, X Wang, T Liu, Q Ye, B Wu, G Hu, S Yang… - Biotechnology for …, 2021 - Springer
Background Zymomonas mobilis is a natural ethanologen with many desirable
characteristics, making it an ideal platform for future biorefineries. Recently, an endogenous …

Contribution of cellulose synthesis, formation of fibrils and their entanglement to the self‐flocculation of Zymomonas mobilis

J Xia, CG Liu, XQ Zhao, Y Xiao… - Biotechnology and …, 2018 - Wiley Online Library
Due to the unique Entner‐Doudoroff pathway, Zymomonas mobilis has been acknowledged
as a potential host to be engineered for biorefinery to produce biofuels and biobased …

Thermophilic and Alkaliphilic Bacillus licheniformis YNP5-TSU as an Ideal Candidate for 2,3-Butanediol Production

J O'Hair, Q Jin, D Yu, N Poe, H Li, S Thapa… - Acs sustainable …, 2020 - ACS Publications
2, 3-Butanediol (2, 3-BD) is an important platform chemical that can be produced
biologically. It is currently fermented below 40° C using mesophilic strains at acid to neutral …

[HTML][HTML] Biosensor-assisted CRISPRi high-throughput screening to identify genetic targets in Zymomonas mobilis for high D-lactate production

Q Peng, W Bao, B Geng, S Yang - Synthetic and Systems Biotechnology, 2024 - Elsevier
Lactate is an important monomer for the synthesis of poly-lactate (PLA), which is a substitute
for the petrochemical plastics. To achieve the goal of high lactate titer, rate, and yield for …

Separation, recovery and upgrading of 2, 3‐butanediol from fermentation broth

T Rajale, X Yang, EJ Judge, CM Moore… - Biofuels, Bioproducts …, 2023 - Wiley Online Library
Butanediol (BDO) is a bio‐derived building block available from biomass through
biochemical methods in high titers (> 120 g L− 1) making it an attractive target for production …

Improved 2, 3-butanediol yield and productivity from lignocellulose biomass hydrolysate in metabolically engineered Enterobacter aerogenes

DG Kim, SW Yoo, M Kim, JK Ko, Y Um, MK Oh - Bioresource technology, 2020 - Elsevier
We previously engineered Enterobacter aerogenes for glucose and xylose co-utilization and
2, 3-butanediol production. Here, strain EMY-22 was further engineered to improve the 2, 3 …

Multiomic fermentation using chemically defined synthetic hydrolyzates revealed multiple effects of lignocellulose-derived inhibitors on cell physiology and xylose …

Y Zhang, JM Vera, D Xie, J Serate… - Frontiers in …, 2019 - frontiersin.org
Utilization of both C5 and C6 sugars to produce biofuels and bioproducts is a key goal for
the development of integrated lignocellulosic biorefineries. Previously we found that …

Deciphering molecular mechanism underlying self-flocculation of Zymomonas mobilis for robust production

LY Cao, YF Yang, X Zhang, YH Chen… - Applied and …, 2022 - Am Soc Microbiol
Zymomonas mobilis metabolizes sugar anaerobically through the Entner-Doudoroff pathway
with less ATP generated for lower biomass accumulation to direct more sugar for product …

A reconciliation of genome-scale metabolic network model of Zymomonas mobilis ZM4

H Nouri, H Fouladiha, H Moghimi, SA Marashi - Scientific Reports, 2020 - nature.com
Zymomonas mobilis ZM4 has recently been used for a variety of biotechnological purposes.
To rationally enhance its metabolic performance, a reliable genome-scale metabolic …

Engineering of Klebsiella oxytoca for production of 2,3‐butanediol using mixed sugars derived from lignocellulosic hydrolysates

JW Cha, SH Jang, YJ Kim, YK Chang… - GCB Bioenergy, 2020 - Wiley Online Library
Butanediol (2, 3‐BDO) is a promising bulk chemical owing to its high potential in industrial
applications. Here, we engineered Klebsiella oxytoca for the economic production of 2, 3 …