Comprehensive identification of protein disulfide bonds with pepsin/trypsin digestion, Orbitrap HCD and Spectrum Identification Machine
Disulfide bonds (SS) are post-translational modifications important for the proper folding and
stabilization of many cellular proteins with therapeutic uses, including antibodies and other …
stabilization of many cellular proteins with therapeutic uses, including antibodies and other …
Trans-reduction of cerebral small vessel disease proteins by notch-derived EGF-like sequences
NMP Cartee, SJ Lee, KZ Young, X Zhang… - International Journal of …, 2022 - mdpi.com
Cysteine oxidation states of extracellular proteins participate in functional regulation and in
disease pathophysiology. In the most common inherited dementia, cerebral autosomal …
disease pathophysiology. In the most common inherited dementia, cerebral autosomal …
Rapid Disulfide Mapping in Peptides and Proteins by meta-Chloroperoxybenzoic Acid (mCPBA) Oxidation and Tandem Mass Spectrometry
Disulfide bonds are a class of important post-translational modifications that play important
roles in modulating the structures and functions of proteins. Therefore, the mapping of …
roles in modulating the structures and functions of proteins. Therefore, the mapping of …
Large-scale qualitative and quantitative assessment of dityrosine crosslinking omics in response to endogenous and exogenous hydrogen peroxide in Escherichia …
X Zhou, F Liu, N Li, Y Zhang - Antioxidants, 2023 - mdpi.com
Excessive hydrogen peroxide causes oxidative stress in cells. The oxidation of two tyrosine
residues in proteins can generate o, o′-dityrosine, a putative biomarker for protein …
residues in proteins can generate o, o′-dityrosine, a putative biomarker for protein …
Xolik: finding cross-linked peptides with maximum paired scores in linear time
Motivation Cross-linking technique coupled with mass spectrometry (MS) is widely used in
the analysis of protein structures and protein-protein interactions. In order to identify cross …
the analysis of protein structures and protein-protein interactions. In order to identify cross …
Characterization of post-translational modifications in full-length human BMP-1 confirms the presence of a rare vicinal disulfide linkage in the catalytic domain and …
CW Hung, T Koudelka, C Anastasi, A Becker… - Journal of …, 2016 - Elsevier
Abstract Bone morphogenetic protein 1 (BMP-1) is an essential metalloproteinase to trigger
extracellular matrix assembly and organogenesis. Previous structural studies on the …
extracellular matrix assembly and organogenesis. Previous structural studies on the …
Quantifying missing (phospho) proteome regions with the broad-specificity protease subtilisin
H Gonczarowska-Jorge, S Loroch, M Dell'Aica… - Analytical …, 2017 - ACS Publications
Despite huge efforts to map the human proteome using mass spectrometry the overall
sequence coverage achieved to date is still below 50%. Reasons for missing areas of the …
sequence coverage achieved to date is still below 50%. Reasons for missing areas of the …
Carboxypeptidase Y assisted disulfide-bond identification with linearized database search
J Qiang, Z Xu, Y Li, H Wang, Y Zhang - Analytical Chemistry, 2021 - ACS Publications
A disulfide bond is an important protein post-translational modification and plays a key role
in regulating protein oxidation status, protein structure, and stability. Analysis of a disulfide …
in regulating protein oxidation status, protein structure, and stability. Analysis of a disulfide …
Comprehensive workflow for mapping disulfide linkages including free thiols and error checking by on-line UV-induced precolumn reduction and spiked control
CM Kuo, SY Wei, SH Du, JL Lin, CH Chu… - Analytical …, 2020 - ACS Publications
Mapping highly complicated disulfide linkages and free thiols via liquid chromatography–
tandem mass spectrometry (LC–MS2) is challenging because of the difficulties in optimizing …
tandem mass spectrometry (LC–MS2) is challenging because of the difficulties in optimizing …
Large conformation shifts of Vibrio cholerae VqmA dimer in the absence of target DNA provide insight into DNA-binding mechanisms of LuxR-type receptors
H Wu, M Li, C Peng, Y Yin, H Guo, W Wang… - Biochemical and …, 2019 - Elsevier
Quorum sensing regulates the biofilm formation and expression of virulence factors in Vibrio
cholerae, an obligate human pathogen that continues to imperil human health. Cytoplasmic …
cholerae, an obligate human pathogen that continues to imperil human health. Cytoplasmic …