No cell lives in a vacuum, and the molecular interactions between cells define most
phenotypes. Transcriptomics provides rich information to infer cell–cell interactions and …

Perturbation of mitochondrial function can trigger a host of cellular responses that seek to
restore cellular metabolism, cytosolic proteostasis, and redox homeostasis. In some cases …

Highlights•Tumor-immune CCIs can be investigated using scRNA-seq and ST
strategies.•CCI studies elucidate fundamental mechanisms in disease state.•Open-source …

The proteome, or collection of proteoforms expressed in a biological system, is dynamic and
heterogeneous. As our appreciation for the complexity of the proteome has evolved, so have …

Characterizing the protein constituents of a specific organelle and protein neighbors of a
protein of interest (POI) is essential for understanding the function and state of the organelle …

In recent years, it has become increasingly clear that many nuclear membrane-less
compartments have liquid-like properties and may form through the physicochemical …

Proximity labeling proteomics (PLP) strategies are powerful approaches to yield snapshots
of protein neighborhoods. Here, we describe a multiscale PLP method with adjustable …

In situ profiling of subcellular proteomics in primary living systems, such as native tissues or
clinic samples, is crucial for understanding life processes and diseases, yet challenging due …

Although APEX2-mediated proximity labeling has been extensively implemented for
studying RNA subcellular localization in live cells, the biotin-phenoxyl radical used for …

Liquid-liquid phase separation (LLPS) has emerged as a crucial mechanism for cellular
compartmentalization. One prominent example of this is the stress granule. Found in various …