Natural and designer cellulosomes: A potential tool for enhancing microbial additive-mediated lignocellulosic agricultural waste composting

UE Senadheera, J Jayasanka, D Udayanga… - Bioresource Technology …, 2023 - Elsevier
Lignocellulosic agricultural waste composting is a cost-effective approach for remedying
such waste. The complex, recalcitrant nature of lignocellulosic agricultural waste hinders its …

Research progress of multi-enzyme complexes based on the design of scaffold protein

X Wang, Y Jiang, H Liu, H Yuan, D Huang… - Bioresources and …, 2023 - Springer
Multi-enzyme complexes designed based on scaffold proteins are a current topic in
molecular enzyme engineering. They have been gradually applied to increase the …

Phase‐separated multienzyme compartmentalization for terpene biosynthesis in a prokaryote

Y Wang, M Liu, Q Wei, W Wu, Y He, J Gao… - Angewandte …, 2022 - Wiley Online Library
Liquid–liquid phase separation (LLPS) forms biomolecular condensates or coacervates in
cells. Metabolic enzymes can form phase‐separated subcellular compartments that enrich …

Engineered Artificial Membraneless Organelles in Saccharomyces cerevisiae To Enhance Chemical Production

P Zhou, H Liu, X Meng, H Zuo, M Qi… - Angewandte Chemie …, 2023 - Wiley Online Library
Microbial cell factories provide a green and sustainable opportunity to produce value‐added
products from renewable feedstock. However, the leakage of toxic or volatile intermediates …

Metabolic pathway assembly using docking domains from type I cis-AT polyketide synthases

X Sun, Y Yuan, Q Chen, S Nie, J Guo, Z Ou… - Nature …, 2022 - nature.com
Engineered metabolic pathways in microbial cell factories often have no natural
organization and have challenging flux imbalances, leading to low biocatalytic efficiency …

Programming an orthogonal self-assembling protein cascade based on reactive peptide–protein pairs for in vitro enzymatic trehalose production

X Chen, X Chen, L Zhu, W Liu… - Journal of Agricultural and …, 2022 - ACS Publications
Trehalose is an important rare sugar that protects biomolecules against environmental
stress. We herein introduce a dual enzyme cascade strategy that regulates the proportion of …

Closed-loop system driven by ADP phosphorylation from pyrophosphate affords equimolar transformation of ATP to 3′-phosphoadenosine-5′-phosphosulfate

R Xu, Y Wang, H Huang, X Jin, J Li, G Du, Z Kang - ACS Catalysis, 2021 - ACS Publications
3′-Phosphoadenosine-5′-phosphosulfate (PAPS) is a universal sulfate group donor for
all biological sulfation reactions in living organisms. Ambitions to biomanufacture sulfate …

Self-assembled recombinant camel serum albumin nanoparticles-encapsulated hemin with peroxidase-like activity for colorimetric detection of hydrogen peroxide and …

J Zhang, Q Xu, W Pei, L Cai, X Yu, H Jiang… - International Journal of …, 2021 - Elsevier
The construction of enzyme mimics using protein protection layers possesses advantages of
high biocompatibility and superior catalytic activity, which is desirable for biomedical …

Co-localizing key pathway enzymes by protein scaffold to enhance geraniol production in Escherichia coli

L Xiao, X Wang, J Zhang, Y Zhou, F Wang… - Industrial Crops and …, 2023 - Elsevier
The key point of geraniol as an isoprenoid biofuel is the yield, however, the toxicity problem
caused by the accumulation of intermediate metabolites hinders the high yield of geraniol. A …

Synthetic Protein Scaffolds for Improving R-(−)-Linalool Production in Escherichia coli

J Wu, X Wang, L Xiao, F Wang… - Journal of Agricultural …, 2021 - ACS Publications
R-(−)-Linalool is widely used in the pharmaceutical, agrochemical, and fragrance industries;
however, its applications are limited owing to low yield and high cost of production. To …