The transcription cycle of RNA polymerase II (RNAPII) is governed at multiple points by
opposing actions of cyclin-dependent kinases (CDKs) and protein phosphatases, in a …

Eukaryotic RNA polymerases (RNA pols) transcriptional processes have been extensively
investigated, and the structural analysis of eukaryotic RNA pols has been explored …

The study of low-abundance proteins is a challenge to discovery-based proteomics. Mass
spectrometry (MS) applications, such as thermal proteome profiling (TPP), face specific …

Summary RNA polymerase II (Pol II)-mediated transcription in metazoans requires precise
regulation. RNA Pol II-associated protein 2 (RPAP2) was previously identified to transport …

In eukaryotes, cellular RNAs are produced by three nuclear RNA polymerases (RNAPI, II,
and III), which are multisubunit complexes. They share structural and functional features …

Rtr1 is an RNA polymerase II (RNA pol II) CTD-phosphatase that influences gene
expression during the transition from transcription initiation to elongation and during …

RNA pol II assembly occurs in the cytoplasm before translocation of the enzyme to the
nucleus. Affecting this assembly influences mRNA transcription in the nucleus and mRNA …

Background Promoter-proximal pausing of RNA polymerase II (RNAPII) is a critical step for
the precise regulation of gene expression. Despite the apparent close relationship between …

Abstract RNA polymerase II (RNAPII) is an essential machinery for catalyzing mRNA
synthesis and controlling cell fate in eukaryotes. Although the structure and function of …

The modified E. coli biotin ligase BirA* was the first developed for proximity labeling of
proteins (BioID). However, it has low activity at temperatures below 37° C, which reduces its …