Characterization of BioID tagging systems in budding yeast and exploring the interactome of the Ccr4-Not complex.

J Pfannenstein, M Tyryshkin, ME Gulden, EH Doud… - bioRxiv, 2024 - biorxiv.org
The modified E. coli biotin ligase BirA* was the first developed for proximity labeling of
proteins (BioID). However, it has low activity at temperatures below 37° C, which reduces its …

RPAP2 regulates a transcription initiation checkpoint by prohibiting assembly of preinitiation complex

X Chen, Y Qi, X Wang, Z Wang, L Wang, A Song, J Li… - bioRxiv, 2021 - biorxiv.org
Abstract RNA polymerase II (Pol II)-mediated transcription in metazoan requires precise
regulation. RNA polymerase II-associated protein 2 (RPAP2) was previously identified to …

[图书][B] Mass Spectrometry Based Elucidation of Transcriptional and Protein Folding Stress

DA Baldwin - 2023 - search.proquest.com
Abstract RNA Polymerase II (RNAPII) plays a pivotal role in cellular homeostasis due to its
role of RNA transcription and gene expression. Many protein-protein interactions (PPIs) are …

Rrp6p/Rrp47p constitutes an independent nuclear turnover system of mature small non-coding RNAs in Saccharomyces cerevisiae

A Chaudhuri, S Das, M Banerjea, B Das - bioRxiv, 2020 - biorxiv.org
In Saccharomyces cerevisiae, the nuclear exosome/Rrp6p/TRAMP participates in the 3'-end
processing of several precursor non-coding RNAs. Here we demonstrate that the depletion …

RNA polymerase II assembly and mRNA decay regulation are mediated and interconnected via CTD Ser5P phosphatase Rtr1 in Saccharomyces cerevisiae

AI Garrido-Godino, A Cuevas-Bermúdez… - bioRxiv, 2020 - biorxiv.org
Rtr1 is an RNA pol II CTD-phosphatase that influences gene expression by acting during the
transition from transcription initiation to elongation, and during transcription termination. Rtr1 …