Statistical approaches to maximize recombinant protein expression in Escherichia coli: a general review
CP Papaneophytou, G Kontopidis - Protein expression and purification, 2014 - Elsevier
The supply of many valuable proteins that have potential clinical or industrial use is often
limited by their low natural availability. With the modern advances in genomics, proteomics …
limited by their low natural availability. With the modern advances in genomics, proteomics …
Reliable protein production in a Pseudomonas fluorescens expression system
DM Retallack, H Jin, L Chew - Protein expression and purification, 2012 - Elsevier
A bottleneck to product development can be reliable expression of active target protein. A
wide array of recombinant proteins in development, including an ever growing number of …
wide array of recombinant proteins in development, including an ever growing number of …
[HTML][HTML] PlantPepDB: A manually curated plant peptide database
Plants produce an array of peptides as part of their innate defense mechanism against
pathogens. The potential use of these peptides for various therapeutic purposes is …
pathogens. The potential use of these peptides for various therapeutic purposes is …
[HTML][HTML] Immobilized metal-affinity chromatography protein-recovery screening is predictive of crystallographic structure success
R Choi, A Kelley, D Leibly… - … Section F: Structural …, 2011 - scripts.iucr.org
The recombinant expression of soluble proteins in Escherichia coli continues to be a major
bottleneck in structural genomics. The establishment of reliable protocols for the …
bottleneck in structural genomics. The establishment of reliable protocols for the …
QconCATs: design and expression of concatenated protein standards for multiplexed protein quantification
DM Simpson, RJ Beynon - Analytical and bioanalytical chemistry, 2012 - Springer
Abstract Systems biology requires knowledge of the absolute amounts of proteins in order to
model biological processes and simulate the effects of changes in specific model …
model biological processes and simulate the effects of changes in specific model …
A screening strategy for heterologous protein expression in Escherichia coli with the highest return of investment
B Pacheco, L Crombet, P Loppnau, D Cossar - Protein expression and …, 2012 - Elsevier
Heterologous protein expression in Escherichia coli is commonly used to obtain
recombinant proteins for a variety of downstream applications. However, many proteins are …
recombinant proteins for a variety of downstream applications. However, many proteins are …
A relationship between mRNA expression levels and protein solubility in E. coli
GG Tartaglia, S Pechmann, CM Dobson… - Journal of molecular …, 2009 - Elsevier
Each step in the process of gene expression, from the transcription of DNA into mRNA to the
folding and posttranslational modification of proteins, is regulated by complex cellular …
folding and posttranslational modification of proteins, is regulated by complex cellular …
[HTML][HTML] High-throughput protein expression using a combination of ligation-independent cloning (LIC) and infrared fluorescent protein (IFP) detection
H Dortay, UM Akula, C Westphal, M Sittig… - PloS one, 2011 - journals.plos.org
Protein expression in heterologous hosts for functional studies is a cumbersome effort. Here,
we report a superior platform for parallel protein expression in vivo and in vitro. The platform …
we report a superior platform for parallel protein expression in vivo and in vitro. The platform …
Recombinant human Tat-Hsp70-2: A tool for neuroprotection
P Cappelletti, E Binda, M Tunesi, D Albani… - Protein Expression and …, 2017 - Elsevier
Human Hsp70-2 is a chaperone expressed mainly in the nervous system. Up to now, no
study has reported on the recombinant expression of this important human chaperone …
study has reported on the recombinant expression of this important human chaperone …
[HTML][HTML] Effect of restricted dissolved oxygen on expression of Clostridium difficile toxin A subunit from E. coli
AK Sharma, J Phue, E Khatipov, N Dalal… - Scientific reports, 2020 - nature.com
The repeating unit of the C. difficile Toxin A (rARU, also known as CROPS [combined
repetitive oligopeptides]) C-terminal region, was shown to elicit protective immunity against …
repetitive oligopeptides]) C-terminal region, was shown to elicit protective immunity against …