Statistical approaches to maximize recombinant protein expression in Escherichia coli: a general review

CP Papaneophytou, G Kontopidis - Protein expression and purification, 2014 - Elsevier
The supply of many valuable proteins that have potential clinical or industrial use is often
limited by their low natural availability. With the modern advances in genomics, proteomics …

Reliable protein production in a Pseudomonas fluorescens expression system

DM Retallack, H Jin, L Chew - Protein expression and purification, 2012 - Elsevier
A bottleneck to product development can be reliable expression of active target protein. A
wide array of recombinant proteins in development, including an ever growing number of …

[HTML][HTML] PlantPepDB: A manually curated plant peptide database

D Das, M Jaiswal, FN Khan, S Ahamad, S Kumar - Scientific reports, 2020 - nature.com
Plants produce an array of peptides as part of their innate defense mechanism against
pathogens. The potential use of these peptides for various therapeutic purposes is …

[HTML][HTML] Immobilized metal-affinity chromatography protein-recovery screening is predictive of crystallographic structure success

R Choi, A Kelley, D Leibly… - … Section F: Structural …, 2011 - scripts.iucr.org
The recombinant expression of soluble proteins in Escherichia coli continues to be a major
bottleneck in structural genomics. The establishment of reliable protocols for the …

QconCATs: design and expression of concatenated protein standards for multiplexed protein quantification

DM Simpson, RJ Beynon - Analytical and bioanalytical chemistry, 2012 - Springer
Abstract Systems biology requires knowledge of the absolute amounts of proteins in order to
model biological processes and simulate the effects of changes in specific model …

A screening strategy for heterologous protein expression in Escherichia coli with the highest return of investment

B Pacheco, L Crombet, P Loppnau, D Cossar - Protein expression and …, 2012 - Elsevier
Heterologous protein expression in Escherichia coli is commonly used to obtain
recombinant proteins for a variety of downstream applications. However, many proteins are …

A relationship between mRNA expression levels and protein solubility in E. coli

GG Tartaglia, S Pechmann, CM Dobson… - Journal of molecular …, 2009 - Elsevier
Each step in the process of gene expression, from the transcription of DNA into mRNA to the
folding and posttranslational modification of proteins, is regulated by complex cellular …

[HTML][HTML] High-throughput protein expression using a combination of ligation-independent cloning (LIC) and infrared fluorescent protein (IFP) detection

H Dortay, UM Akula, C Westphal, M Sittig… - PloS one, 2011 - journals.plos.org
Protein expression in heterologous hosts for functional studies is a cumbersome effort. Here,
we report a superior platform for parallel protein expression in vivo and in vitro. The platform …

Recombinant human Tat-Hsp70-2: A tool for neuroprotection

P Cappelletti, E Binda, M Tunesi, D Albani… - Protein Expression and …, 2017 - Elsevier
Human Hsp70-2 is a chaperone expressed mainly in the nervous system. Up to now, no
study has reported on the recombinant expression of this important human chaperone …

[HTML][HTML] Effect of restricted dissolved oxygen on expression of Clostridium difficile toxin A subunit from E. coli

AK Sharma, J Phue, E Khatipov, N Dalal… - Scientific reports, 2020 - nature.com
The repeating unit of the C. difficile Toxin A (rARU, also known as CROPS [combined
repetitive oligopeptides]) C-terminal region, was shown to elicit protective immunity against …