Review on methods of solving the refractive index–thickness coupling problem in digital holographic microscopy of biological cells

G Dardikman, NT Shaked - Optics Communications, 2018 - Elsevier
Digital holographic microscopy is a thriving imaging modality that attracted considerable
research interest in quantitative biological cell imaging due to its ability to not only create …

Generalized cell morphological parameters based on interferometric phase microscopy and their application to cell life cycle characterization

P Girshovitz, NT Shaked - Biomedical optics express, 2012 - opg.optica.org
We present analysis tools which are formulated using wide-field interferometric phase
microscopy measurements, and show their ability to uniquely quantify the life cycle of live …

Automated analysis of individual sperm cells using stain‐free interferometric phase microscopy and machine learning

SK Mirsky, I Barnea, M Levi, H Greenspan… - Cytometry Part …, 2017 - Wiley Online Library
Currently, the delicate process of selecting sperm cells to be used for in vitro fertilization
(IVF) is still based on the subjective, qualitative analysis of experienced clinicians using non …

[HTML][HTML] Adipocyte stiffness increases with accumulation of lipid droplets

N Shoham, P Girshovitz, R Katzengold, NT Shaked… - Biophysical journal, 2014 - cell.com
Adipogenesis and increase in fat tissue mass are mechanosensitive processes and hence
should be influenced by the mechanical properties of adipocytes. We evaluated subcellular …

Quantitative phase imaging (QPI) in neuroscience

C Hu, G Popescu - IEEE Journal of Selected Topics in Quantum …, 2018 - ieeexplore.ieee.org
Quantitative phase imaging (QPI) is an emerging label-free modality that attracts significant
interest in biomedicine in general and neuroscience in particular. Based on the principle of …

[HTML][HTML] Multimodal holographic microscopy: distinction between apoptosis and oncosis

J Balvan, A Krizova, J Gumulec, M Raudenska… - PloS one, 2015 - journals.plos.org
Identification of specific cell death is of a great value for many scientists. Predominant types
of cell death can be detected by flow-cytometry (FCM). Nevertheless, the absence of cellular …

[HTML][HTML] High accuracy label-free classification of single-cell kinetic states from holographic cytometry of human melanoma cells

M Hejna, A Jorapur, JS Song, RL Judson - Scientific reports, 2017 - nature.com
Digital holographic cytometry (DHC) permits label-free visualization of adherent cells.
Dozens of cellular features can be derived from segmentation of hologram-derived images …

Integral refractive index imaging of flowing cell nuclei using quantitative phase microscopy combined with fluorescence microscopy

G Dardikman, YN Nygate, I Barnea… - Biomedical optics …, 2018 - opg.optica.org
We suggest a new multimodal imaging technique for quantitatively measuring the integral
(thickness-average) refractive index of the nuclei of live biological cells in suspension. For …

Dual-channel low-coherence interferometry and its application to quantitative phase imaging of fingerprints

H Gabai, NT Shaked - Optics express, 2012 - opg.optica.org
We introduce an off-axis, wide-field, low-coherence and dual-channel interferometric
imaging system, which is based on a simple-to-align, common-path interferometer. The …

Label-free microscopy: A non-invasive new tool to assess gametes and embryo quality

M Rubessa, MB Wheeler - Theriogenology, 2020 - Elsevier
In PubMed, it is possible to find more than 40,000 papers on embryo evaluation in various
species. However, there is no consensus or gold standard method on how to assess their …