Alginate as an immobilization material for MAb production via encapsulated hybridoma cells

SM Selimoglu, M Elibol - Critical reviews in biotechnology, 2010 - Taylor & Francis
Alginate has been widely used in various applications since its first extraction. What makes
this biopolymer useful is its high biocompatibility and humid gelation conditions. Both of …

Alginate bead fabrication and encapsulation of living cells under centrifugally induced artificial gravity conditions

S Haeberle, L Naegele, R Burger, F Stetten… - Journal of …, 2008 - Taylor & Francis
This study presents a novel method for the direct, centrifugally induced fabrication of small,
Ca2+-hardened alginate beads at polymer-tube micronozzles. The bead diameter can …

Nutrient capsules maintain tear film homeostasis for human corneal lenticule transplantation

J Zhao, Z Zhang, F Xia, G Li, Q Saiding, L Xiang… - Chemical Engineering …, 2022 - Elsevier
Corneal lenticule transplantation can be applied in the correction of refractive errors and
various corneal diseases. However, current techniques to preserve cornea are limited due to …

Immobilized enzymes and cells in poly(N-vinyl caprolactam)-based hydrogels: Preparation, properties, and applications in biotechnology and medicine

EA Markvicheva, SV Kuptsova, TY Mareeva… - Applied biochemistry …, 2000 - Springer
A one-step mild method for entrapping animal cells and enzymes in macroporous composite
poly (N-vinyl caprolactam)-calcium alginate (PVCL-CaAlg) hydrogels is described. Some …

Validation of a novel perfusion bioreactor system in cancer research

J Stojkovska, J Zvicer, M Milivojević, I Petrović… - Hemijska …, 2020 - doiserbia.nb.rs
Development of drugs is a complex, time-and cost-consuming process due to the lack of
standardized and reliable characterization techniques and models. Traditionally, drug …

Stability of antibody productivity is improved when hybridoma cells are entrapped in calcium alginate beads

GM Lee, BO Palsson - Biotechnology and bioengineering, 1993 - Wiley Online Library
Loss of monoclonal antibody (MAb) productivity in long‐term, free‐suspended cell culture is
often attributed to the appearance of a nonproducing population of hybridoma cell (NP) in …

Cell culture conditions determine the enhancement of specific monoclonal antibody productivity of calcium alginate‐entrapped S3H5/γ2bA2 hybridoma cells

GM Lee, AS Chuck, BO Palsson - Biotechnology and …, 1993 - Wiley Online Library
Immobilization offers several intrinsic advantages over free suspension cultures for the
production of monoclonal antibodies. An important advantage of immobilization is the …

Batik Dye Decolorization by Immobilized Biomass of Aspergillus sp

RS Dewi, A Mumpuni, NI Tsabitah - IOP Conference Series: Earth …, 2020 - iopscience.iop.org
The rapidity of the batik industry in the Former Residence of Banyumas raises problems.
Wastewater that discharged into upstream causes accumulation in downstream up to …

A thermosensitive gel matrix for bioreactor-assisted in-cell NMR of nucleic acids and proteins

M Dzurov, Š Pospíšilová, M Krafčíková… - Journal of biomolecular …, 2023 - Springer
Introducing the flow through the bioreactor has revolutionized in-cell NMR spectroscopy by
prolonging the measurement time available to acquire spectral information about …

Enhancement of monoclonal antibody production by immobilized hybridoma cell culture with hyperosmolar medium

SY Park, GM Lee - Biotechnology and bioengineering, 1995 - Wiley Online Library
To determine the effect of hyperosmotic stress on the monoclonal antibody (MAb) production
by calcium‐alginate‐immobilized S3H5/γ2bA2 hybridoma cells, the osmolalities of medium …