Genomes have complex three-dimensional architectures. The recent convergence of
genetic, biochemical, biophysical, and cell biological methods has uncovered several …

The four-dimensional nucleome (4DN) consortium studies the architecture of the genome
and the nucleus in space and time. We summarize progress by the consortium and highlight …

Eukaryotic genomes are packaged into a 3-dimensional structure in the nucleus. Current
methods for studying genome-wide structure are based on proximity ligation. However, this …

The folding of genomic DNA from the beads-on-a-string-like structure of nucleosomes into
higher-order assemblies is crucially linked to nuclear processes. Here we calculate 3D …

Recent progress in whole-genome mapping and imaging technologies has enabled the
characterization of the spatial organization and folding of the genome in the nucleus. In …

At the nuclear periphery, associations of chromatin with the nuclear lamina through lamina-
associated domains (LADs) aid functional organization of the genome. We review the …

Organization of the genome into euchromatin and heterochromatin appears to be
evolutionarily conserved and relatively stable during lineage differentiation. In an effort to …

Several general principles of global 3D genome organization have recently been
established, including non-random positioning of chromosomes and genes in the cell …

There is a need for methods that can image chromosomes with genome-wide coverage, as
well as greater genomic and optical resolution. We introduce OligoFISSEQ, a suite of three …

Rapidly developing technologies have recently fueled an exciting era of discovery in the
field of chromosome structure and nuclear organization. In addition to chromosome …