New technologies in high-throughput screening have significantly increased throughput and
reduced assay volumes. Key advances over the past few years include new fluorescence …

The mathematical concept of fluorescence correlation spectroscopy (FCS) 1 (1) emerged
from quasi-elastic light scattering (QELS) spectroscopy (2) in the early 1970s. Compared to …

Single-molecule Förster resonance energy transfer (smFRET) is increasingly being used to
determine distances, structures, and dynamics of biomolecules in vitro and in vivo. However …

G-protein-coupled receptors (GPCRs) are key signaling proteins that mostly function as
monomers, but for several receptors constitutive dimer formation has been described and in …

Single-molecule FRET (smFRET) has become a mainstream technique for studying
biomolecular structural dynamics. The rapid and wide adoption of smFRET experiments by …

In the recent decade, single-molecule (sm) spectroscopy has come of age and is providing
important insight into how biological molecules function. So far our view of protein function is …

We describe a simple approach and present a straightforward numerical algorithm to
compute the best fit shot-noise limited proximity ratio histogram (PRH) in single-molecule …

A general strategy to identify and quantify sample molecules in dilute solution employing a
new spectroscopic method for data registration and specific burst analysis denoted as multi …

Upon transfer from strongly denaturing to native conditions, proteins undergo a collapse that
either precedes folding or occurs simultaneously with it. This collapse is similar to the well …

By combining the photoswitching and localization of individual fluorophores with
spectroscopy on the single molecule level, we demonstrate simultaneous multicolor imaging …