Proteins carry out a wide variety of catalytic, regulatory, signalling and structural functions in
living systems. Following their assembly on ribosomes and throughout their lifetimes, most …

Protein semisynthesis—defined herein as the assembly of a protein from a combination of
synthetic and recombinant fragments—is a burgeoning field of chemical biology that has …

Regulation of protein function through thiol-based redox switches plays an important role in
the response and adaptation to local and global changes in the cellular levels of reactive …

The ability to manipulate the chemical composition of proteins and peptides has been
central to the development of improved polypeptide-based therapeutics and has enabled …

Protein trans-splicing (PTS) by split inteins has found widespread use in chemical biology
and biotechnology. Herein, we describe the use of a consensus design approach to …

This review adds the bilin-binding phytochromes to the Chemical Reviews thematic issue
“Optogenetics and Photopharmacology”. The work is structured into two parts. We first …

Protein splicing is a posttranslational modification where intervening proteins (inteins)
cleave themselves from larger precursor proteins and ligate their flanking polypeptides …

Here, to overcome many limitations accompanying current available methods to detect
protein-protein interactions (PPIs), we develop a live cell method called Split Intein-Mediated …

Split inteins are privileged molecular scaffolds for the chemical modification of proteins.
Though efficient for in vitro applications, these polypeptide ligases have not been utilized for …

Naturally split inteins have found widespread use in chemical biology due to their ability to
drive the ligation of separately expressed polypeptides through a process termed protein …