[HTML][HTML] Current status and future perspectives of supports and protocols for enzyme immobilization

FTT Cavalcante, ALG Cavalcante, IG de Sousa… - Catalysts, 2021 - mdpi.com
The market for industrial enzymes has witnessed constant growth, which is currently around
7% a year, projected to reach $10.5 billion in 2024. Lipases are hydrolase enzymes …

[HTML][HTML] Mimicking natural strategies to create multi-environment enzymatic reactors: From natural cell compartments to artificial polyelectrolyte reactors

M Omidvar, J Zdarta, SB Sigurdardóttir, M Pinelo - Biotechnology Advances, 2022 - Elsevier
Engineering microenvironments for sequential enzymatic reactions has attracted specific
interest within different fields of research as an effective strategy to improve the catalytic …

Reprogramming cellular metabolism to increase the efficiency of microbial cell factories

Q Ding, L Liu - Critical Reviews in Biotechnology, 2023 - Taylor & Francis
Recent studies are increasingly focusing on advanced biotechnological tools, self-adjusting
smart microorganisms, and artificial intelligent networks, to engineer microorganisms with …

Nanoindentation Response of 3D Printed PEGDA Hydrogels in a Hydrated Environment

MH Khalili, CJ Williams, C Micallef… - ACS Applied Polymer …, 2023 - ACS Publications
Hydrogels are commonly used materials in tissue engineering and organ-on-chip devices.
This study investigated the nanomechanical properties of monolithic and multilayered poly …

Leveraging the Power of Enzymes in Engineered Dead and Living Materials

MR Shannon, B Zhou… - Advanced Functional …, 2024 - Wiley Online Library
Significant advances are being made in the incorporation of enzymes into functional
materials, which leverage their inherent substrate specificity, efficient catalytic activity, and …

Advanced strategies in high-throughput droplet screening for enzyme engineering

W Yan, X Li, D Zhao, M Xie, T Li, L Qian, C Ye… - Biosensors and …, 2023 - Elsevier
Enzymes, as biocatalysts, play a cumulatively important role in environmental purification
and industrial production of chemicals and pharmaceuticals. However, natural enzymes are …

Effect of multimodularity and spatial organization of glycoside hydrolases on catalysis

V Barba-Cedillo, CY Montanier - Essays in Biochemistry, 2023 - portlandpress.com
The wide diversity among the carbohydrate-active enzymes (CAZymes) reflects the equally
broad versatility in terms of composition and chemicals bonds found in the plant cell wall …

Rational Design of Artificial Biofilms as Sustainable Supports for Whole‐Cell Catalysis Through Integrating Extra‐and Intracellular Catalysis

H Dong, W Zhang, S Zhou, H Ying, P Wang - ChemSusChem, 2022 - Wiley Online Library
Biofilms are promising candidates for sustainable bioprocessing applications. This work
presents a rational design of biofilm catalysts by integrating extra‐and intracellular catalysis …

Engineered Escherichia coli Consortia Function in a Programmable Pattern for Multiple Enzymatic Biosynthesis

W Zhang, H Dong, X Wang, L Zhang… - … Applied Materials & …, 2023 - ACS Publications
Coordinating microbial consortia to realize complex synthetic pathways is an area of great
interest in the rapidly growing field of biomanufacturing. This work presents a programmable …

Protein engineering approach to enhance activity assays of mono-ADP-ribosyltransferases through proximity

A Galera-Prat, J Alaviuhkola, HI Alanen… - … , Design and Selection, 2022 - academic.oup.com
Human mono-ADP-ribosylating PARP enzymes have been linked to several clinically
relevant processes and many of these PARPs have been suggested as potential drug …