Cryobiology of in vitro-derived bovine embryos

SP Leibo, NM Loskutoff - Theriogenology, 1993 - Elsevier
The capability to produce bovine embryos from ovarian oocytes by in vitro methods of
maturation, fertilization and culture has progressed very rapidly during the past few years …

Ultrastructure of bovine embryos developed from in vitro–matured and–fertilized oocytes: Comparative morphological evaluation of embryos cultured either in serum …

H Abe, S Yamashita, T Itoh, T Satoh… - Molecular …, 1999 - Wiley Online Library
The ultrastructure of bovine embryos developed from in vitro‐matured and‐fertilized oocytes,
cocultured with bovine cumulus/granulosa cells either in a serum‐free medium (IVMD101) …

Effects of oocyte quality, oxygen tension, embryo density, cumulus cells and energy substrates on cleavage and morula/blastocyst formation of bovine embryos

NK Khurana, H Niemann - Theriogenology, 2000 - Elsevier
Various factors, such as quality of the oocyte, oxygen tension, embryo density, and kind of
energy substrate during in vitro production of embryos may affect the rate of preimplantation …

Factors affecting bovine embryo development in synthetic oviduct fluid following oocyte maturation and fertilization in vitro

C Carolan, P Lonergan, A Van Langendonckt… - Theriogenology, 1995 - Elsevier
Employing a total of 3465 bovine oocytes this study was aimed at improving the efficiency of
bovine embryo production under defined and undefined conditions. Following in vitro …

In vitro maturation, fertilization and culture to blastocysts of bovine oocytes in protein-free media

J Eckert, H Niemann - Theriogenology, 1995 - Elsevier
This study examined the role of protein supplementation at the various steps of the in vitro
production of bovine embryos derived from two different morphological categories of COC …

Effect of culture media on embryo development from prepubertal goat IVM-IVF oocytes

D Izquierdo, P Villamediana, MT Paramio - Theriogenology, 1999 - Elsevier
Experiments were carried out to develop and improve in vitro culture systems for IVM-IVF
prepubertal goat oocytes. Cumulus oocyte complexes (COC) were obtained by slicing …

In vitro culture of bovine IVM-IVF embryos: cooperative interaction among embryos and the role of growth factors

CL Keefer, SL Stice, AM Paprocki, P Golueke - Theriogenology, 1994 - Elsevier
The objective of this study was to determine whether there is a cooperative interaction
among bovine embryos during in vitro culture. Furthermore, culture medium was …

A serum-free, cell-free culture system for development of bovine one-cell embryos up to blastocyst stage with improved viability

M Shamsuddin, B Larsson, H Gustafsson… - Theriogenology, 1994 - Elsevier
With the aim of developing a serum-free, cell-free culture system for embryo development, in
vitro-matured (IVM) and-fertilized (IVF) bovine oocytes were cultured in TCM 199 with the …

Effect of various growth-promoting factors on preimplantation bovine embryo development in vitro

MR Flood, TL Gage, TD Bunch - Theriogenology, 1993 - Elsevier
One-cell bovine embryos produced by in vitro oocyte maturation (IVM) and in vitro
fertilization (IVF) were cultured in chemically defined medium (CDM) to which the following …

Vascular endothelial growth factor (VEGF) promotes the early development of bovine embryo in the presence of cumulus cells

H Luo, K Kimura, M Aoki, M Hirako - Journal of veterinary medical …, 2002 - jstage.jst.go.jp
Three experiments were conducted to determine the effect of Vascular Endothelial Growth
Factor (VEGF) on bovine embryonic development in vitro. Human recombinant VEGF165 …