We have constructed a genome-saturating mutant library of the human gastric pathogen
Helicobacter pylori. Microarray tracking of transposon mutants (MATT) allowed us to map the …

Escherichia coli ST131 is a globally disseminated, multidrug resistant clone responsible for
a high proportion of urinary tract and bloodstream infections. The rapid emergence and …

The pathways in Escherichia coli and (largely by analogy) S. enterica remain the paradigm
of bacterial lipid synthetic pathways, although recently considerable diversity among …

Chloroplasts are the defining organelle of photoautotrophic plant cells. Photosynthetic light
reactions and electron transport are the functions of an elaborate thylakoid membrane …

Gene essentiality is typically determined by assessing the viability of the corresponding
mutant cells, but this definition fails to account for the ability of cells to adaptively evolve to …

The selective pressures leading to the evolution and maintenance of virulence in the case of
facultative pathogens are quite unclear. For example, Escherichia coli, a commensal of the …

Members of the large superclass of P-loop GTPases share a core domain with a conserved
three-dimensional structure. In eukaryotes, these proteins are implicated in various crucial …

Our information about the gene content of organisms continues to grow as more genomes
are sequenced and gene products are characterized. Sequence-based annotation efforts …

Under laboratory conditions 80% of yeast genes seem not to be essential for viability. This
raises the question of what the mechanistic basis for dispensability is, and whether it is the …

ABSTRACT A successful transition of Listeria monocytogenes from the extracellular to the
intracellular environment requires a precise adaptation response to conditions encountered …