[HTML][HTML] Identification of essential genes of the periodontal pathogen Porphyromonas gingivalis
BA Klein, EL Tenorio, DW Lazinski, A Camilli… - BMC genomics, 2012 - Springer
Background Porphyromonas gingivalis is a Gram-negative anaerobic bacterium associated
with periodontal disease onset and progression. Genetic tools for the manipulation of …
with periodontal disease onset and progression. Genetic tools for the manipulation of …
[HTML][HTML] Improved detection of Escherichia coli and coliform bacteria by multiplex PCR
Background The presence of coliform bacteria is routinely assessed to establish the
microbiological safety of water supplies and raw or processed foods. Coliforms are a group …
microbiological safety of water supplies and raw or processed foods. Coliforms are a group …
Genome‐scale gene/reaction essentiality and synthetic lethality analysis
PF Suthers, A Zomorrodi, CD Maranas - Molecular systems biology, 2009 - embopress.org
Synthetic lethals are to pairs of non‐essential genes whose simultaneous deletion prohibits
growth. One can extend the concept of synthetic lethality by considering gene groups of …
growth. One can extend the concept of synthetic lethality by considering gene groups of …
CRISPR tools to control gene expression in bacteria
A Vigouroux, D Bikard - Microbiology and Molecular Biology …, 2020 - Am Soc Microbiol
SUMMARY CRISPR-Cas systems have been engineered as powerful tools to control gene
expression in bacteria. The most common strategy relies on the use of Cas effectors …
expression in bacteria. The most common strategy relies on the use of Cas effectors …
[HTML][HTML] Genome-wide essential gene identification in Streptococcus sanguinis
A clear perception of gene essentiality in bacterial pathogens is pivotal for identifying drug
targets to combat emergence of new pathogens and antibiotic-resistant bacteria, for …
targets to combat emergence of new pathogens and antibiotic-resistant bacteria, for …
Evolution of chromosome organization driven by selection for reduced gene expression noise
The distribution of genes on eukaryotic chromosomes is nonrandom, but the reasons behind
this are not well understood. The commonly observed clustering of essential genes is a case …
this are not well understood. The commonly observed clustering of essential genes is a case …
Bacillus subtilis and Escherichia coli essential genes and minimal cell factories after one decade of genome engineering
M Juhas, DR Reuß, B Zhu… - Microbiology, 2014 - microbiologyresearch.org
Investigation of essential genes, besides contributing to understanding the fundamental
principles of life, has numerous practical applications. Essential genes can be exploited as …
principles of life, has numerous practical applications. Essential genes can be exploited as …
Whole proteome analysis of post-translational modifications: applications of mass-spectrometry for proteogenomic annotation
While bacterial genome annotations have significantly improved in recent years, techniques
for bacterial proteome annotation (including post-translational chemical modifications, signal …
for bacterial proteome annotation (including post-translational chemical modifications, signal …
Systematic Mutagenesis of the Escherichia coli Genome
Y Kang, T Durfee, JD Glasner, Y Qiu… - Journal of …, 2004 - Am Soc Microbiol
ABSTRACT A high-throughput method has been developed for the systematic mutagenesis
of the Escherichia coli genome. The system is based on in vitro transposition of a modified …
of the Escherichia coli genome. The system is based on in vitro transposition of a modified …