Expansion microscopy (ExM) is a newly developed super-resolution technique, allowing
visualization of biological targets at nanoscale resolution on conventional fluorescence …

Super-resolution microscopy is a series of imaging techniques that bypass the diffraction
limit of resolution. Since the 1990s, optical approaches, such as single-molecular …

Expansion microscopy enables nanoimaging with conventional microscopes by physically
and isotropically magnifying preserved biological specimens embedded in a crosslinked …

Cellular organization and functions encompass multiple scales in vivo. Emerging high-plex
imaging technologies are limited in resolving subcellular biomolecular features. Expansion …

A key feature of excitatory synapses is the existence of subsynaptic protein nanoclusters
(NCs) whose precise alignment across the cleft in a transsynaptic nanocolumn influences …

Proteins are densely packed in cells and tissues, where they form complex nanostructures.
Expansion microscopy (ExM) variants have been used to separate proteins from each other …

Expansion microscopy (ExM), by physically enlarging specimens in an isotropic fashion,
enables nanoimaging on standard light microscopes. Key to existing ExM protocols is the …

The postsynaptic density (PSD) of excitatory synapses is built from a wide variety of
scaffolding proteins, receptors, and signaling molecules that collectively orchestrate synaptic …

Growing evidence suggests a remarkable diversity and complexity in the molecular
composition of synapses, forming the basis for the brain to execute complex behaviors …

To understand how the molecular machinery of synapses works, it is essential to determine
an inventory of synaptic proteins at a subsynaptic resolution. Nevertheless, synaptic proteins …