Innovations to culturing the uncultured microbial majority

WH Lewis, G Tahon, P Geesink, DZ Sousa… - Nature Reviews …, 2021 - nature.com
Despite the surge of microbial genome data, experimental testing is important to confirm
inferences about the cell biology, ecological roles and evolution of microorganisms. As the …

Adaptive genetic traits in pelagic freshwater microbes

MC Chiriac, M Haber… - Environmental …, 2023 - Wiley Online Library
Pelagic microbes have adopted distinct strategies to inhabit the pelagial of lakes and
oceans and can be broadly categorized in two groups: free‐living, specialized oligotrophs …

Genome‐resolved viral and cellular metagenomes revealed potential key virus‐host interactions in a deep freshwater lake

Y Okazaki, Y Nishimura, T Yoshida… - Environmental …, 2019 - Wiley Online Library
Metagenomics has dramatically expanded the known virosphere, but freshwater viral
diversity and their ecological interaction with hosts remain poorly understood. Here, we …

Long-read-resolved, ecosystem-wide exploration of nucleotide and structural microdiversity of lake bacterioplankton genomes

Y Okazaki, S Nakano, A Toyoda, H Tamaki - Msystems, 2022 - Am Soc Microbiol
Reconstruction of metagenome-assembled genomes (MAGs) has become a fundamental
approach in microbial ecology. However, a MAG is hardly complete and overlooks genomic …

Microbial communities of the Laurentian Great Lakes reflect connectivity and local biogeochemistry

SF Paver, RJ Newton… - Environmental …, 2020 - Wiley Online Library
Summary The Laurentian Great Lakes are a vast, interconnected freshwater system
spanning strong physicochemical gradients, thus constituting a powerful natural laboratory …

Paleoecological evidence for a multi-trophic regime shift in a perialpine lake (Lake Joux, Switzerland)

MÈ Monchamp, R Bruel, V Frossard, S McGowan… - Anthropocene, 2021 - Elsevier
Freshwater ecosystems are under new and increasing threats from anthropogenic change.
Ability to detect and predict consequences of environmental perturbations on ecosystem …

Stratification of microbiomes during the holomictic period of Lake Fuxian, an alpine monomictic lake

P Xing, Y Tao, J Luo, L Wang, B Li… - Limnology and …, 2020 - Wiley Online Library
In warm monomictic lakes, the hypolimnion is important for accumulating and decomposing
organic matter derived from surface production, and the regenerated nutrients will be …

[HTML][HTML] Microdiversity and phylogeographic diversification of bacterioplankton in pelagic freshwater systems revealed through long-read amplicon sequencing

Y Okazaki, S Fujinaga, MM Salcher, C Callieri… - Microbiome, 2021 - Springer
Background Freshwater ecosystems are inhabited by members of cosmopolitan
bacterioplankton lineages despite the disconnected nature of these habitats. The lineages …

[HTML][HTML] Effects of habitat partitioning on the distribution of bacterioplankton in deep lakes

N Salmaso - Frontiers in Microbiology, 2019 - frontiersin.org
In deep lakes, many investigations highlighted the existence of exclusive groups of bacteria
adapted to deep oxygenated and hypoxic and anoxic hypolimnia. Nevertheless, the extent …

Anoxygenic phototrophic Chloroflexota member uses a Type I reaction center

JM Tsuji, NA Shaw, S Nagashima, JJ Venkiteswaran… - bioRxiv, 2020 - biorxiv.org
Chlorophyll-based phototrophy is performed using quinone and/or Fe-S type reaction
centers,. Unlike oxygenic phototrophs, where both reaction center classes are used in …