Applications of CRISPR–Cas in agriculture and plant biotechnology
The prokaryote-derived CRISPR–Cas genome editing technology has altered plant
molecular biology beyond all expectations. Characterized by robustness and high target …
molecular biology beyond all expectations. Characterized by robustness and high target …
Recent advances in crop transformation technologies
Agriculture is experiencing a technological inflection point in its history, while also facing
unprecedented challenges posed by human population growth and global climate changes …
unprecedented challenges posed by human population growth and global climate changes …
[HTML][HTML] Heritable transgene-free genome editing in plants by grafting of wild-type shoots to transgenic donor rootstocks
Generation of stable gene-edited plant lines using clustered regularly interspaced short
palindromic repeats (CRISPR)–CRISPR-associated protein 9 (Cas9) requires a lengthy …
palindromic repeats (CRISPR)–CRISPR-associated protein 9 (Cas9) requires a lengthy …
[HTML][HTML] Highly efficient heritable genome editing in wheat using an RNA virus and bypassing tissue culture
Genome editing provides novel strategies for improving plant traits but mostly relies on
conventional plant genetic transformation and regeneration procedures, which can be …
conventional plant genetic transformation and regeneration procedures, which can be …
VIGS goes viral: how VIGS transforms our understanding of plant science
Virus-induced gene silencing (VIGS) has developed into an indispensable approach to
gene function analysis in a wide array of species, many of which are not amenable to stable …
gene function analysis in a wide array of species, many of which are not amenable to stable …
Potato virus X vector-mediated DNA-free genome editing in plants
H Ariga, S Toki, K Ishibashi - Plant and Cell Physiology, 2020 - academic.oup.com
Genome editing technology is important for plant science and crop breeding. Genome-
edited plants prepared using general CRISPR-Cas9 methods usually contain foreign DNA …
edited plants prepared using general CRISPR-Cas9 methods usually contain foreign DNA …
[HTML][HTML] Genome editing in cereal crops: an overview
JM Matres, J Hilscher, A Datta, V Armario-Nájera… - Transgenic …, 2021 - Springer
Genome-editing technologies offer unprecedented opportunities for crop improvement with
superior precision and speed. This review presents an analysis of the current state of …
superior precision and speed. This review presents an analysis of the current state of …
Efficient Cas9 multiplex editing using unspaced sgRNA arrays engineering in a Potato virus X vector
M Uranga, V Aragonés, S Selma… - The Plant …, 2021 - Wiley Online Library
Systems based on the clustered, regularly interspaced, short palindromic repeat (CRISPR)
and CRISPR‐associated proteins (Cas) have revolutionized genome editing in many …
and CRISPR‐associated proteins (Cas) have revolutionized genome editing in many …
[HTML][HTML] Virus-induced gene editing and its applications in plants
C Zhang, S Liu, X Li, R Zhang, J Li - International Journal of Molecular …, 2022 - mdpi.com
CRISPR/Cas-based genome editing technologies, which allow the precise manipulation of
plant genomes, have revolutionized plant science and enabled the creation of germplasms …
plant genomes, have revolutionized plant science and enabled the creation of germplasms …
[HTML][HTML] Control of plant viruses by CRISPR/Cas system-mediated adaptive immunity
Y Cao, H Zhou, X Zhou, F Li - Frontiers in Microbiology, 2020 - frontiersin.org
Plant diseases caused by invading plant viruses pose serious threats to agricultural
production in the world, and the antiviral engineering initiated by molecular biotechnology …
production in the world, and the antiviral engineering initiated by molecular biotechnology …