[HTML][HTML] Protein scaffolds: a tool for multi-enzyme assembly

S Gad, S Ayakar - Biotechnology Reports, 2021 - Elsevier
The synthesis of complex molecules using multiple enzymes simultaneously in one reaction
vessel has rapidly emerged as a new frontier in the field of bioprocess technology. However …

Recent advances in laccase activity assays: a crucial challenge for applications on complex substrates

E Martin, P Dubessay, E Record, F Audonnet… - Enzyme and Microbial …, 2023 - Elsevier
Despite being one of the first enzymes discovered in 1883, the determination of laccase
activity remains a scientific challenge, and a barrier to the full use of laccase as a biocatalyst …

MEP pathway products allosterically promote monomerization of deoxy-D-xylulose-5-phosphate synthase to feedback-regulate their supply

X Di, D Ortega-Alarcon, R Kakumanu… - Plant …, 2023 - cell.com
Isoprenoids are a very large and diverse family of metabolites required by all living
organisms. All isoprenoids derive from the double-bond isomers isopentenyl diphosphate …

Monitoring protein conformational changes using fluorescent nanoantennas

SG Harroun, D Lauzon, MC Ebert, A Desrosiers… - Nature …, 2022 - nature.com
Understanding the relationship between protein structural dynamics and function is crucial
for both basic research and biotechnology. However, methods for studying the fast dynamics …

Electrochemical Biosensing of L-DOPA Using Tyrosinase Immobilized on Carboxymethyl Starch-Graft-Polyaniline@MWCNTs Nanocomposite

F Mollamohammadi, H Faridnouri, EN Zare - Biosensors, 2023 - mdpi.com
The electrochemical behavior of the immobilized tyrosinase (Tyrase) on a modified glassy
carbon electrode with carboxymethyl starch-graft-polyaniline/multi-walled carbon nanotubes …

Applications of isothermal titration calorimetry in pure and applied research from 2016 to 2020

RJ Falconer, B Schuur… - Journal of Molecular …, 2021 - Wiley Online Library
The last 5 years have seen a series of advances in the application of isothermal titration
microcalorimetry (ITC) and interpretation of ITC data. ITC has played an invaluable role in …

Complexation of anthocyanins, betalains and carotenoids with biopolymers: An approach to complexation techniques and evaluation of binding parameters

F Rocha, J de Paula Rezende… - Food Research …, 2023 - Elsevier
Natural pigments are bioactive compounds that can present health-promoting bioactivities in
the human body. Due to their strong coloring properties, these compounds have been …

Advances in computational methods to discover new NS2B-NS3 inhibitors useful against dengue and Zika viruses

IJ dos Santos Nascimento… - Current Topics in …, 2022 - ingentaconnect.com
The Flaviviridae virus family consists of the genera Hepacivirus, Pestivirus, and Flavivirus,
with approximately 70 viral types that use arthropods as vectors. Among these diseases …

An on-line detection system for screening small molecule inhibitors of α-Amylase and α-Glucosidase in Prunus mume

X Nan, W Jia, Y Zhang, H Wang, Z Lin… - Journal of Chromatography …, 2022 - Elsevier
High-throughput screening of inhibitors from natural products is an efficient approach to
target key enzymes in diabetes progression. In this study, an on-line detection system was …

A novel CRISPR/Cas14a1-Exo III aptasensor for melamine detection coupled with systematically studied binding mechanism of truncated aptamer

P Ma, H Guo, Y Zhang, Z Wang - Sensors and Actuators B: Chemical, 2023 - Elsevier
In this work, the aptamer of melamine was truncated and mutated. Based on isothermal
titration calorimetry, circular dichroism and molecular dynamics, the thermodynamic …