[HTML][HTML] Insights into drought stress signaling in plants and the molecular genetic basis of cotton drought tolerance

T Mahmood, S Khalid, M Abdullah, Z Ahmed… - Cells, 2019 - mdpi.com
Drought stress restricts plant growth and development by altering metabolic activity and
biological functions. However, plants have evolved several cellular and molecular …

[HTML][HTML] Construct design for CRISPR/Cas-based genome editing in plants

MM Hassan, Y Zhang, G Yuan, K De, JG Chen… - Trends in Plant …, 2021 - cell.com
CRISPR construct design is a key step in the practice of genome editing, which includes
identification of appropriate Cas proteins, design and selection of guide RNAs (gRNAs), and …

Optimizing the CRISPR/Cas9 system for genome editing in grape by using grape promoters

C Ren, Y Liu, Y Guo, W Duan, P Fan, S Li… - Horticulture …, 2021 - academic.oup.com
The efficacy of the CRISPR/Cas9 system in grapevine (Vitis vinifera L.) has been
documented, but the optimization of this system, as well as CRISPR/Cas9-mediated …

Genome editing for plant research and crop improvement

X Zhan, Y Lu, JK Zhu, JR Botella - Journal of Integrative Plant …, 2021 - Wiley Online Library
The advent of clustered regularly interspaced short palindromic repeat (CRISPR) has had a
profound impact on plant biology, and crop improvement. In this review, we summarize the …

[HTML][HTML] Metabolic engineering of plant secondary metabolites: prospects and its technological challenges

A Mipeshwaree Devi, K Khedashwori Devi… - Frontiers in Plant …, 2023 - frontiersin.org
Plants produce a wide range of secondary metabolites that play vital roles for their primary
functions such as growth, defence, adaptations or reproduction. Some of the plant …

[HTML][HTML] High efficacy full allelic CRISPR/Cas9 gene editing in tetraploid potato

IE Johansen, Y Liu, B Jørgensen, EP Bennett… - Scientific Reports, 2019 - nature.com
CRISPR/Cas9 editing efficacies in tetraploid potato were highly improved through the use of
endogenous potato U6 promoters. Highly increased editing efficiencies in the Granular …

[HTML][HTML] CRISPR-Cas9 system for plant genome editing: Current approaches and emerging developments

JAV Montecillo, LL Chu, H Bae - Agronomy, 2020 - mdpi.com
Targeted genome editing using CRISPR-Cas9 has been widely adopted as a genetic
engineering tool in various biological systems. This editing technology has been in the …

[HTML][HTML] An Efficient Genetic Transformation and CRISPR/Cas9-Based Genome Editing System for Moso Bamboo (Phyllostachys edulis)

B Huang, R Zhuo, H Fan, Y Wang, J Xu, K Jin… - Frontiers in plant …, 2022 - frontiersin.org
Moso bamboo (Phyllostachys edulis) is the most important monopodial bamboo species
worldwide. Without a genetic transformation system, it is difficult to verify the functions of …

[HTML][HTML] Hairy CRISPR: genome editing in plants using hairy root transformation

AS Kiryushkin, EL Ilina, ED Guseva, K Pawlowski… - Plants, 2021 - mdpi.com
CRISPR/Cas-mediated genome editing is a powerful tool of plant functional genomics. Hairy
root transformation is a rapid and convenient approach for obtaining transgenic roots. When …

[HTML][HTML] Heritable gene editing using FT mobile guide RNAs and DNA viruses

J Lei, P Dai, Y Li, W Zhang, G Zhou, C Liu, X Liu - Plant Methods, 2021 - Springer
Background The virus-induced genome editing (VIGE) system can be used to quickly
identify gene functions and generate knock-out libraries as an alternative to the virus …