A highly sensitive, real-time centrifugal microfluidic chip for multiplexed detection based on isothermal amplification

X Dong, Z Tang, X Jiang, Q Fu, D Xu, L Zhang, X Qiu - Talanta, 2024 - Elsevier
A real-time centrifugal microfluidic chip with a companion analyzer was developed for highly
sensitive, multiplexed nucleic acid detection based on RPA (recombinase polymerase …

One-pot, ultrasensitive, and multiplex detection of SARS-CoV-2 genes utilizing self-priming hairpin-mediated isothermal amplification

Y Li, T Kang, HG Park - Biosensors and Bioelectronics, 2023 - Elsevier
The global pandemic resulting from the severe acute respiratory syndrome coronavirus 2
(SARS-CoV-2) and its emerging variants highlights the need for convenient and accurate …

An HFman probe-based reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for HIV-1 detection

X Zhang, H Li, Z Liu, Y Zhao, Y Zeng, Y Dong… - Molecular and cellular …, 2022 - Elsevier
Loop-mediated isothermal amplification (LAMP) is suitable for the development of a rapid
and cost-effective nucleic acid technique for point of care (POC) applications. However …

[HTML][HTML] Low-temperature loop-mediated isothermal amplification operating at physiological temperature

D Nam, S Kim, JH Kim, S Lee, D Kim, J Son, D Kim… - Biosensors, 2023 - mdpi.com
Loop-mediated isothermal amplification (LAMP) is one of the most widely used isothermal
amplification technologies in molecular diagnostics. However, LAMP operates at a high …

[HTML][HTML] Rapid, sensitive, and visual detection of Clonorchis sinensis with an RPA-CRISPR/Cas12a-based dual readout portable platform

T Huang, L Li, J Li, X Li, S Li, X Wang, N Zhang… - International Journal of …, 2023 - Elsevier
Clonorchis sinensis is a food-borne parasite that parasitizes the liver and bile ducts of
humans and many animals. This parasite exerts a high burden due to diverse hepatobiliary …

[HTML][HTML] Recent Uses of Paper Microfluidics in Isothermal Nucleic Acid Amplification Tests

J Reynolds, RS Loeffler, PJ Leigh, HA Lopez, JY Yoon - Biosensors, 2023 - mdpi.com
Isothermal nucleic acid amplification tests have recently gained popularity over polymerase
chain reaction (PCR), as they only require a constant temperature and significantly simplify …

Cas14a1-advanced LAMP for ultrasensitive and visual Pathogen diagnostic

Q Wang, Y Ren, T Meng, X Yang, L Lu, H Yang, H Hou… - Talanta, 2024 - Elsevier
Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas enzymes have
been widely applied for biosensor development, combined with various isothermal …

Rapid and simple colorimetric detection of quiescent Colletotrichum in harvested fruit using reverse transcriptional loop-mediated isothermal amplification (RT-LAMP) …

M Kaur, K Ayarnah, D Duanis-Assaf, N Alkan, E Eltzov - Talanta, 2023 - Elsevier
Anthracnose, caused by the fungus Colletotrichum gloeosporioides, is one of the major
causes of postharvest decay of fruits and vegetables. Detection of the pathogen at an early …

[HTML][HTML] Quantitative mRNA expression measurement at home

S Pandey, SS McCoy, T Stobdan, D Sahoo - Scientific Reports, 2024 - nature.com
Abstract mRNA measurement is dominated by RT-PCR, which requires expensive
laboratory equipment and personnel with advanced degrees. Loop-mediated isothermal …

[HTML][HTML] A Comparative Study on Visual Detection of Mycobacterium tuberculosis by Closed Tube Loop-Mediated Isothermal Amplification: Shedding Light on the Use …

A Neshani, H Zare, H Sadeghian, H Safdari… - Diagnostics, 2023 - mdpi.com
Loop-mediated isothermal amplification is a promising candidate for the rapid detection of
Mycobacterium tuberculosis. However, the high potential for carry-over contamination is the …