[HTML][HTML] Merging automation and fundamental discovery into the design–build–test–learn cycle of nontraditional microbes
Major advances toward a bio-based industry enabled cost-efficient bioproduction of multiple
chemicals, yet successful industrial processes are relatively scarce and limited to the use of …
chemicals, yet successful industrial processes are relatively scarce and limited to the use of …
[HTML][HTML] Challenges and opportunities in bringing nonbiological atoms to life with synthetic metabolism
R Haas, PI Nikel - Trends in Biotechnology, 2023 - cell.com
The relatively narrow spectrum of chemical elements within the microbial 'biochemical
palate'limits the reach of biotechnology, because several added-value compounds can only …
palate'limits the reach of biotechnology, because several added-value compounds can only …
A nonconventional archaeal fluorinase identified by in silico mining for enhanced fluorine biocatalysis
Fluorinases, the only enzymes known to catalyze the transfer of fluorine to an organic
molecule, are essential catalysts for the biological synthesis of valuable organofluorines …
molecule, are essential catalysts for the biological synthesis of valuable organofluorines …
[HTML][HTML] Integrated rational and evolutionary engineering of genome-reduced Pseudomonas putida strains promotes synthetic formate assimilation
Formate is a promising, water-soluble C1 feedstock for biotechnology that can be efficiently
produced from CO 2—but formatotrophy has been engineered in only a few industrially …
produced from CO 2—but formatotrophy has been engineered in only a few industrially …
Role of the CrcB transporter of Pseudomonas putida in the multi‐level stress response elicited by mineral fluoride
The presence of mineral fluoride (F−) in the environment has both a geogenic and
anthropogenic origin, and the halide has been described to be toxic in virtually all living …
anthropogenic origin, and the halide has been described to be toxic in virtually all living …
[HTML][HTML] A synthetic C2 auxotroph of Pseudomonas putida for evolutionary engineering of alternative sugar catabolic routes
Acetyl-coenzyme A (AcCoA) is a metabolic hub in virtually all living cells, serving as both a
key precursor of essential biomass components and a metabolic sink for catabolic pathways …
key precursor of essential biomass components and a metabolic sink for catabolic pathways …
Engineering non-haem iron enzymes for enantioselective C(sp3)–F bond formation via radical fluorine transfer
In recent years there has been a surge in the development of methods for the synthesis of
organofluorine compounds. However, enzymatic methods for C–F bond formation have …
organofluorine compounds. However, enzymatic methods for C–F bond formation have …
From Rags to Riches: Exploiting the Calvin‐Benson‐Bassham Cycle for Biomanufacturing
Industrial chemical production largely relies on fossil fuels, resulting in the unavoidable
release of carbon dioxide (CO2) into the atmosphere. The concept of a circular carbon …
release of carbon dioxide (CO2) into the atmosphere. The concept of a circular carbon …
[HTML][HTML] Enzymatic synthesis of mono-and trifluorinated alanine enantiomers expands the scope of fluorine biocatalysis
M Nieto-Domínguez, A Sako… - Communications …, 2024 - nature.com
Fluorinated amino acids serve as an entry point for establishing new-to-Nature chemistries
in biological systems, and novel methods are needed for the selective synthesis of these …
in biological systems, and novel methods are needed for the selective synthesis of these …
Identification of Genes Essential for Fluorination and Sulfamylation within the Nucleocidin Gene Clusters of Streptomyces calvus and Streptomyces virens
The gene cluster in Streptomyces calvus associated with the biosynthesis of the fluoro‐and
sulfamyl‐metabolite nucleocidin was interrogated by systematic gene knockouts. Out of the …
sulfamyl‐metabolite nucleocidin was interrogated by systematic gene knockouts. Out of the …