Fluorescence lifetime imaging (FLIM) uses the fact that the fluorescence lifetime of a
fluorophore depends on its molecular environment but not on its concentration. Molecular …

Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the
environment and interaction of fluorescent probes. It can report on photophysical events that …

To a certain extent, all cellular, physiological, and pathological phenomena that occur in
cells are accompanied by ionic changes. The development of techniques allowing the …

In fluorescence microscopy, the fluorescence emission can be characterised not only by
intensity and position, but also by lifetime, polarization and wavelength. Fluorescence …

Biochemical estimation of NADH concentration is a useful method for monitoring cellular
metabolism, because the NADH/NAD+ reduction-oxidation pair is crucial for electron …

The room temperature dynamics of single sulforhodamine 101 molecules dispersed on a
glass surface are investigated on two different time scales with near-field optics. On the 10-2 …

A frequency domain fluorescence lifetime imaging microscope (FLIM) has been developed.
A continuous wave laser excitation source of an epi-illumination fluorescence microscope is …

An emerging theme in cell biology is that cell surface receptors need to be considered as
part of supramolecular complexes of proteins and lipids facilitating specific receptor …

The functional characteristics of fluorescent probes used for imaging and measuring
dynamic processes in living cells are reviewed. Initial consideration is given to general …

Förster's resonance energy transfer (FRET) can be used to study protein-protein interactions
in living cells. Numerous methods to measure FRET have been devised and implemented; …