The discovery of the CRISPR/Cas genome-editing system has revolutionized our
understanding of the plant genome. CRISPR/Cas has been used for over a decade to …

Precise replacement of an allele with an elite allele controlling an important agronomic trait
in a predefined manner by gene editing technologies is highly desirable in crop …

CRISPR/Cas-based genome editing technologies, which allow the precise manipulation of
plant genomes, have revolutionized plant science and enabled the creation of germplasms …

Highlights●Adapting new nucleases and improving established ones expand the
CRISPR/Cas toolbox.●Optimized base editors enlarge editing windows and enable CG …

The clustered regularly interspaced short palindromic repeats (CRISPR)/associated protein
9 system (Cas9) has been used at length to optimize multiple aspects of germplasm …

Adenine base editors (ABEs) are valuable, precise genome editing tools in plants. In recent
years, the highly promising ADENINE BASE EDITOR8e (ABE8e) was reported for efficient A …

Genetic modifications are made through diverse mutagenesis techniques for crop
improvement programs. Among these mutagenesis tools, the traditional methods involve …

Base editors, such as adenine base editors (ABE) and cytosine base editors (CBE), provide
alternatives for precise genome editing without generating double-strand breaks (DSBs) …

CRISPR/Cas9-based cytosine base editors (CBEs) and adenine base editors (ABEs) can
efficiently mediate C-to-T/G-to-A and A-to-G/T-to-C substitutions, respectively; however …

Following recent developments and refinement, CRISPR-Cas9 gene-editing technology has
become increasingly mature and is being widely used for crop improvement. The application …