The necessity of a specific protospacer adjacent motif (PAM) greatly limited the editing
scope and the application range of Cas9-dependent base editors. Here, we showed that …

Small peptides represent a subset of dark matter in plant proteomes. Through differential
expression patterns and modes of action, small peptides act as important regulators of plant …

A plethora of CRISPR effectors, such as Cas3, Cas9, and Cas12a, are commonly employed
as gene editing tools. Among these, Cas12 effectors developed based on Class II type V …

Histone acetylation is the earliest and most well-characterized of post-translation
modifications. It is mediated by histone acetyltransferases (HAT) and histone deacetylases …

Carotenoids are indispensable to plants and critical components of the human diet. The
carotenoid metabolic pathway is conserved across plant species, but our understanding of …

Cytosine and adenosine base editors (CBE and ABE) have been widely used in plants,
greatly accelerating gene function research and crop breeding. Current base editors can …

Main conclusion The enhancement of CRISPR-Cas gene editing with robust nuclease
activity promotes genetic modification of desirable agronomic traits, such as resistance to …

Maize (Zea mays) is one of the most important food crops in the world with the greatest
global production, and contributes to satiating the demands for human food, animal feed …

Recently, clustered regularly interspaced palindromic repeats (CRISPR)-Cas9 derived
editing tools had significantly improved our ability to make desired changes in the genome …

CRISPR/Cas9 技术是利用RNA 靶向引导Cas9 核酸酶对基因组中的目标基因进行编辑的生物
技术. 近年来, 该技术的多种新型基因编辑器更新迅猛, 编辑效果愈加精细和高效 …