Plant DNA barcoding: from gene to genome
ABSTRACT DNA barcoding is currently a widely used and effective tool that enables rapid
and accurate identification of plant species; however, none of the available loci work across …
and accurate identification of plant species; however, none of the available loci work across …
[HTML][HTML] Library preparation for next generation sequencing: A review of automation strategies
Next generation sequencing is in the process of evolving from a technology used for
research purposes to one which is applied in clinical diagnostics. Recently introduced high …
research purposes to one which is applied in clinical diagnostics. Recently introduced high …
Astrocytic interleukin-3 programs microglia and limits Alzheimer's disease
Communication within the glial cell ecosystem is essential for neuronal and brain health,–.
The influence of glial cells on the accumulation and clearance of β-amyloid (Aβ) and …
The influence of glial cells on the accumulation and clearance of β-amyloid (Aβ) and …
Unconstrained genome targeting with near-PAMless engineered CRISPR-Cas9 variants
Manipulation of DNA by CRISPR-Cas enzymes requires the recognition of a protospacer-
adjacent motif (PAM), limiting target site recognition to a subset of sequences. To remove …
adjacent motif (PAM), limiting target site recognition to a subset of sequences. To remove …
Key innovations and the diversification of Hymenoptera
The order Hymenoptera (wasps, ants, sawflies, and bees) represents one of the most
diverse animal lineages, but whether specific key innovations have contributed to its …
diverse animal lineages, but whether specific key innovations have contributed to its …
Precise cut-and-paste DNA insertion using engineered type VK CRISPR-associated transposases
CJ Tou, B Orr, BP Kleinstiver - Nature Biotechnology, 2023 - nature.com
CRISPR-associated transposases (CASTs) enable recombination-independent, multi-
kilobase DNA insertions at RNA-programmed genomic locations. However, the utility of type …
kilobase DNA insertions at RNA-programmed genomic locations. However, the utility of type …
Transcriptome-wide off-target RNA editing induced by CRISPR-guided DNA base editors
CRISPR–Cas base-editor technology enables targeted nucleotide alterations, and is being
increasingly used for research and potential therapeutic applications,. The most widely used …
increasingly used for research and potential therapeutic applications,. The most widely used …
Engineered CRISPR–Cas12a variants with increased activities and improved targeting ranges for gene, epigenetic and base editing
Broad use of CRISPR–Cas12a (formerly Cpf1) nucleases has been hindered by the
requirement for an extended TTTV protospacer adjacent motif (PAM). To address this …
requirement for an extended TTTV protospacer adjacent motif (PAM). To address this …
The transcriptional landscape of Arabidopsis thaliana pattern-triggered immunity
Plants tailor their metabolism to environmental conditions, in part through the recognition of
a wide array of self and non-self molecules. In particular, the perception of microbial or plant …
a wide array of self and non-self molecules. In particular, the perception of microbial or plant …
Engineered CRISPR prime editors with compact, untethered reverse transcriptases
J Grünewald, BR Miller, RN Szalay… - Nature …, 2023 - nature.com
The CRISPR prime editor PE2 consists of a Streptococcus pyogenes Cas9 nickase
(nSpCas9) fused at its C-terminus to a Moloney murine leukemia virus reverse transcriptase …
(nSpCas9) fused at its C-terminus to a Moloney murine leukemia virus reverse transcriptase …