Emerging droplet microfluidics
Droplet microfluidics generates and manipulates discrete droplets through immiscible
multiphase flows inside microchannels. Due to its remarkable advantages, droplet …
multiphase flows inside microchannels. Due to its remarkable advantages, droplet …
Droplet microfluidics for microbiology: techniques, applications and challenges
Droplet microfluidics has rapidly emerged as one of the key technologies opening up new
experimental possibilities in microbiology. The ability to generate, manipulate and monitor …
experimental possibilities in microbiology. The ability to generate, manipulate and monitor …
Single-cell barcoding and sequencing using droplet microfluidics
Single-cell RNA sequencing has recently emerged as a powerful tool for mapping cellular
heterogeneity in diseased and healthy tissues, yet high-throughput methods are needed for …
heterogeneity in diseased and healthy tissues, yet high-throughput methods are needed for …
Centrifugal micro-channel array droplet generation for highly parallel digital PCR
Stable water-in-oil emulsion is essential to digital PCR and many other bioanalytical
reactions that employ droplets as microreactors. We developed a novel technology to …
reactions that employ droplets as microreactors. We developed a novel technology to …
Droplet-based microfluidic high-throughput screening of heterologous enzymes secreted by the yeast Yarrowia lipolytica
T Beneyton, S Thomas, AD Griffiths, JM Nicaud… - Microbial cell …, 2017 - Springer
Background Droplet-based microfluidics is becoming an increasingly attractive alternative to
microtiter plate techniques for enzymatic high-throughput screening (HTS), especially for …
microtiter plate techniques for enzymatic high-throughput screening (HTS), especially for …
Fluorescence-activated droplet sorting for single-cell directed evolution
Synthetic biology aims to improve human health and the environment by repurposing
biological enzymes for use in practical applications. However, natural enzymes often …
biological enzymes for use in practical applications. However, natural enzymes often …
Droplet-based digital antibiotic susceptibility screen reveals single-cell clonal heteroresistance in an isogenic bacterial population
Since antibiotic resistance is a major threat to global health, recent observations that the
traditional test of minimum inhibitory concentration (MIC) is not informative enough to guide …
traditional test of minimum inhibitory concentration (MIC) is not informative enough to guide …
Spatial confinement toward creating artificial living systems
Lifeforms are regulated by many physicochemical factors, and these factors could be
controlled to play a role in the construction of artificial living systems. Among these factors …
controlled to play a role in the construction of artificial living systems. Among these factors …
Droplet microfluidics and directed evolution of enzymes: an intertwined journey
A Stucki, J Vallapurackal, TR Ward… - Angewandte Chemie …, 2021 - Wiley Online Library
Evolution is essential to the generation of complexity and ultimately life. It relies on the
propagation of the properties, traits, and characteristics that allow an organism to survive in …
propagation of the properties, traits, and characteristics that allow an organism to survive in …
Detection of antibiotics synthetized in microfluidic picolitre-droplets by various actinobacteria
The natural bacterial diversity is regarded as a treasure trove for natural products. However,
accessing complex cell mixtures derived from environmental samples in standardized high …
accessing complex cell mixtures derived from environmental samples in standardized high …