[HTML][HTML] Revealing bacterial cell biology using cryo-electron tomography
Visualizing macromolecules inside bacteria at a high spatial resolution has remained a
challenge owing to their small size and limited resolution of optical microscopy techniques …
challenge owing to their small size and limited resolution of optical microscopy techniques …
Serial Lift-Out: sampling the molecular anatomy of whole organisms
Cryo-focused ion beam milling of frozen-hydrated cells and subsequent cryo-electron
tomography (cryo-ET) has enabled the structural elucidation of macromolecular complexes …
tomography (cryo-ET) has enabled the structural elucidation of macromolecular complexes …
EMDB—the Electron Microscopy Data Bank
Nucleic Acids Research, 2024 - academic.oup.com
Abstract The Electron Microscopy Data Bank (EMDB) is the global public archive of three-
dimensional electron microscopy (3DEM) maps of biological specimens derived from …
dimensional electron microscopy (3DEM) maps of biological specimens derived from …
Visualizing the membrane disruption action of antimicrobial peptides by cryo-electron tomography
EHL Chen, CH Wang, YT Liao, FY Chan… - Nature …, 2023 - nature.com
The abuse of antibiotics has led to the emergence of multidrug-resistant microbial
pathogens, presenting a pressing challenge in global healthcare. Membrane-disrupting …
pathogens, presenting a pressing challenge in global healthcare. Membrane-disrupting …
Mechanisms of E. coli chemotaxis signaling pathways visualized using cryoET and computational approaches
K Hadjidemetriou, S Kaur, CK Cassidy… - Biochemical Society …, 2022 - portlandpress.com
Chemotaxis signaling pathways enable bacteria to sense and respond to their chemical
environment and, in some species, are critical for lifestyle processes such as biofilm …
environment and, in some species, are critical for lifestyle processes such as biofilm …
Ultrastructure of macromolecular assemblies contributing to bacterial spore resistance revealed by in situ cryo-electron tomography
E Bauda, B Gallet, J Moravcova, G Effantin… - Nature …, 2024 - nature.com
Bacterial spores owe their incredible resistance capacities to molecular structures that
protect the cell content from external aggressions. Among the determinants of resistance are …
protect the cell content from external aggressions. Among the determinants of resistance are …
The advent of preventive high-resolution structural histopathology by artificial-intelligence-powered cryogenic electron tomography
JG Galaz-Montoya - Frontiers in Molecular Biosciences, 2024 - frontiersin.org
Advances in cryogenic electron microscopy (cryoEM) single particle analysis have
revolutionized structural biology by facilitating the in vitro determination of atomic-and near …
revolutionized structural biology by facilitating the in vitro determination of atomic-and near …
Lighting the way: how the Vibrio fischeri model microbe reveals the complexity of Earth's “simplest” life forms
Vibrio (Aliivibrio) fischeri's initial rise to fame derived from its alluring production of blue-
green light. Subsequent studies to probe the mechanisms underlying this bioluminescence …
green light. Subsequent studies to probe the mechanisms underlying this bioluminescence …
[HTML][HTML] A dynamic duo: Understanding the roles of FtsZ and FtsA for Escherichia coli cell division through in vitro approaches
Bacteria divide by binary fission. The protein machine responsible for this process is the
divisome, a transient assembly of more than 30 proteins in and on the surface of the …
divisome, a transient assembly of more than 30 proteins in and on the surface of the …
Binding and Functional Folding (BFF): A Physiological Framework for Studying Biomolecular Interactions and Allostery
BD Young, ME Cook, BK Costabile, R Samanta… - Journal of molecular …, 2022 - Elsevier
Abstract EF-hand Ca 2+-binding proteins (CBPs), such as S100 proteins (S100s) and
calmodulin (CaM), are signaling proteins that undergo conformational changes upon …
calmodulin (CaM), are signaling proteins that undergo conformational changes upon …