[HTML][HTML] Revealing bacterial cell biology using cryo-electron tomography

K Khanna, E Villa - Current Opinion in Structural Biology, 2022 - Elsevier
Visualizing macromolecules inside bacteria at a high spatial resolution has remained a
challenge owing to their small size and limited resolution of optical microscopy techniques …

Serial Lift-Out: sampling the molecular anatomy of whole organisms

OH Schiøtz, CJO Kaiser, S Klumpe, DR Morado… - Nature …, 2023 - nature.com
Cryo-focused ion beam milling of frozen-hydrated cells and subsequent cryo-electron
tomography (cryo-ET) has enabled the structural elucidation of macromolecular complexes …

EMDB—the Electron Microscopy Data Bank

Nucleic Acids Research, 2024 - academic.oup.com
Abstract The Electron Microscopy Data Bank (EMDB) is the global public archive of three-
dimensional electron microscopy (3DEM) maps of biological specimens derived from …

Visualizing the membrane disruption action of antimicrobial peptides by cryo-electron tomography

EHL Chen, CH Wang, YT Liao, FY Chan… - Nature …, 2023 - nature.com
The abuse of antibiotics has led to the emergence of multidrug-resistant microbial
pathogens, presenting a pressing challenge in global healthcare. Membrane-disrupting …

Mechanisms of E. coli chemotaxis signaling pathways visualized using cryoET and computational approaches

K Hadjidemetriou, S Kaur, CK Cassidy… - Biochemical Society …, 2022 - portlandpress.com
Chemotaxis signaling pathways enable bacteria to sense and respond to their chemical
environment and, in some species, are critical for lifestyle processes such as biofilm …

Ultrastructure of macromolecular assemblies contributing to bacterial spore resistance revealed by in situ cryo-electron tomography

E Bauda, B Gallet, J Moravcova, G Effantin… - Nature …, 2024 - nature.com
Bacterial spores owe their incredible resistance capacities to molecular structures that
protect the cell content from external aggressions. Among the determinants of resistance are …

The advent of preventive high-resolution structural histopathology by artificial-intelligence-powered cryogenic electron tomography

JG Galaz-Montoya - Frontiers in Molecular Biosciences, 2024 - frontiersin.org
Advances in cryogenic electron microscopy (cryoEM) single particle analysis have
revolutionized structural biology by facilitating the in vitro determination of atomic-and near …

Lighting the way: how the Vibrio fischeri model microbe reveals the complexity of Earth's “simplest” life forms

AN Septer, KL Visick - Journal of Bacteriology, 2024 - Am Soc Microbiol
Vibrio (Aliivibrio) fischeri's initial rise to fame derived from its alluring production of blue-
green light. Subsequent studies to probe the mechanisms underlying this bioluminescence …

[HTML][HTML] A dynamic duo: Understanding the roles of FtsZ and FtsA for Escherichia coli cell division through in vitro approaches

P Radler, M Loose - European Journal of Cell Biology, 2023 - Elsevier
Bacteria divide by binary fission. The protein machine responsible for this process is the
divisome, a transient assembly of more than 30 proteins in and on the surface of the …

Binding and Functional Folding (BFF): A Physiological Framework for Studying Biomolecular Interactions and Allostery

BD Young, ME Cook, BK Costabile, R Samanta… - Journal of molecular …, 2022 - Elsevier
Abstract EF-hand Ca 2+-binding proteins (CBPs), such as S100 proteins (S100s) and
calmodulin (CaM), are signaling proteins that undergo conformational changes upon …