Design of novel enzyme biocatalysts for industrial bioprocess: Harnessing the power of protein engineering, high throughput screening and synthetic biology
Biocatalysts have wider applications in various industries. Biocatalysts are generating
bigger attention among researchers due to their unique catalytic properties like activity …
bigger attention among researchers due to their unique catalytic properties like activity …
Cold active lipases: biocatalytic tools for greener technology
Lipases are enzymes that catalyze the ester bond hydrolysis in triglycerides with the release
of fatty acids, mono-and diglycerides, and glycerol. The microbial lipases account for 400millionmarketsizein2017anditisexpecte …
of fatty acids, mono-and diglycerides, and glycerol. The microbial lipases account for 400millionmarketsizein2017anditisexpecte …
Microbial lipolytic enzymes–promising energy-efficient biocatalysts in bioremediation
Enzymes are biological catalysts that significantly speed up reactions by lowering their
activation energy. Compared to chemical catalysts, enzymatic reactions take place in milder …
activation energy. Compared to chemical catalysts, enzymatic reactions take place in milder …
Microbial production of phytases for combating environmental phosphate pollution and other diverse applications
A Kumar, A Chanderman, M Makolomakwa… - Critical reviews in …, 2016 - Taylor & Francis
Concerns of phosphorus pollution and its impact on environments have driven the
biotechnological development of phytases. Phosphoric acid, inositol phosphate, or inositols …
biotechnological development of phytases. Phosphoric acid, inositol phosphate, or inositols …
Advances in enzyme substrate analysis with capillary electrophoresis
Capillary electrophoresis provides a rapid, cost-effective platform for enzyme and substrate
characterization. The high resolution achievable by capillary electrophoresis enables the …
characterization. The high resolution achievable by capillary electrophoresis enables the …
Directed Evolution of Carbonyl Reductase from Rhodosporidium toruloides and Its Application in Stereoselective Synthesis of tert-Butyl (3R,5S)-6-Chloro-3,5 …
tert-Butyl (3 R, 5 S)-6-chloro-3, 5-dihydroxyhexanoate ((3 R, 5 S)-CDHH) is a key
intermediate of atorvastatin and rosuvastatin synthesis. Carbonyl reductase Rt SCR9 from …
intermediate of atorvastatin and rosuvastatin synthesis. Carbonyl reductase Rt SCR9 from …
Improving alkaline stability of α-L-rhamnosidase from Aspergillus Niger through computational strategy combines with folding free energy and binding free energy
L Li, C Peng, J Gong, X Liu, W Li, Y Zhu, H Ni… - Biochemical Engineering …, 2023 - Elsevier
It is difficult to improve pH stability of enzymes. In the present study, a semi-rational strategy
was validated to improve the alkaline stability of an α-L-rhamnosidase. The strategy …
was validated to improve the alkaline stability of an α-L-rhamnosidase. The strategy …
[HTML][HTML] An insight into ameliorating production, catalytic efficiency, thermostability and starch saccharification of acid-stable α-amylases from acidophiles
D Parashar, T Satyanarayana - Frontiers in bioengineering and …, 2018 - frontiersin.org
Most of the extracellular enzymes of acidophilic bacteria and archaea are stable at acidic pH
with a relatively high thermostability. There is, however, a dearth of information on their acid …
with a relatively high thermostability. There is, however, a dearth of information on their acid …
Ile258Met mutation of Brucella melitensis 7α-hydroxysteroid dehydrogenase significantly enhances catalytic efficiency, cofactor affinity, and thermostability
Z Liu, R Zhang, W Zhang, Y Xu - Applied Microbiology and Biotechnology, 2021 - Springer
Abstract NAD (H)-dependent 7α-hydroxysteroid dehydrogenase catalyzes the oxidation of
chenodeoxycholic acid to 7-oxolithocholic acid. Here, we designed mutations of Ile258 …
chenodeoxycholic acid to 7-oxolithocholic acid. Here, we designed mutations of Ile258 …
Improved catalytic efficiency, thermophilicity, anti-salt and detergent tolerance of keratinase KerSMD by partially truncation of PPC domain
Z Fang, J Zhang, G Du, J Chen - Scientific reports, 2016 - nature.com
The keratinase from Stenotrophomonas maltophilia (KerSMD) is known for its high activity
and pH stability in keratin degradation. However, catalytic efficiency and detergent …
and pH stability in keratin degradation. However, catalytic efficiency and detergent …